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. 2016 Aug 20;11:101–117. doi: 10.1016/j.ebiom.2016.08.027

Fig. 4.

Fig. 4

Trichrome staining of HFD livers and glycemic challenge of WT and Tg-FABP4-RORα4 mice. (A) Formalin-fixed and paraffinized liver tissues of HFD-fed WT and tg/+ mice were sectioned at 0.7 μm-step intervals and stained for (A) collagen using Masson's trichrome staining. Red denotes cytoplasmic staining and the yellow arrowheads indicate collagen staining (blue). Representative images acquired from n = 4 littermate pairs are shown. (B and C) Analysis of mean ± S.E.M. fasting plasma (B) triglyceride and (C) NEFAs from 16 weeks chow-fed WT and tg/+ mice (n = 6 littermate pairs). Significance was calculated using unpaired two-tailed Student's t-test where *P < 0.05. (D) Fasting blood glucose levels from 6 h-fasted WT (white) and tg/+ (black) (n = 10 littermate pairs) mice in the chow study (16 weeks old). (E and F) Blood glucose was measured at various times after intra-peritoneal administration of (E) glucose or (F) insulin (t = 0) on 6 h-fasted WT (black) and tg/+ (red) mice (16 week chow, n = 10 littermate pairs). (G) Fasting blood glucose levels from 6 h-fasted WT (white) and tg/+ (black) mice (n = 5 littermate pairs) in the HFD study. (H and I) Blood glucose was measured at various times after intra-peritoneal administration of (H) glucose or (I) insulin (t = 0) on 6 h-fasted WT (black) and tg/+ (red) mice in the HFD study (n = 5 littermate pairs). Statistical analyses were performed using unpaired two-tailed Student's t-test for panels D and G and two-way ANOVAs with Bonferroni's post-test applied for panels E, F, H and I where *P < 0.05; **P < 0.01;***P < 0.001. (J) Plasma insulin levels of (i) 6 h-fasted (n = 5 littermate pairs) and (ii) overnight fasted WT and tg/+ littermates (n = 8 littermate pairs) from the 32-week chow study. Statistical analysis was performed using unpaired two-tailed Student's t-test but comparisons were not significant. (K) Plasma insulin levels of overnight fasted WT and tg/+ mice in the HFD study (n = 8 littermate pairs). Statistical analysis was performed using unpaired two-tailed Student's t-test but comparisons were not significant.