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. 1995 May;79(5):462–466. doi: 10.1136/bjo.79.5.462

Distribution of a putative endothelial barrier antigen in the ocular and orbital tissues of the rat.

J G Lawrenson 1, M N Ghabriel 1, A R Reid 1, T N Gajree 1, G Allt 1
PMCID: PMC505136  PMID: 7612560

Abstract

BACKGROUND--A rat endothelial barrier antigen (EBA) recognised by a monoclonal antibody has been shown to be expressed strongly by endothelial cells of brain capillaries possessing a blood-brain barrier and only weakly expressed by fenestrated brain vessels. METHODS--In this study immunocytochemical methods for light and electron microscopy were used to study EBA distribution in the eye and orbital tissues of the rat. RESULTS--Blood-ocular barrier vessels in the optic nerve, retina, iris, and some vessels in th choroid and ciliary body were immunopositive for EBA. By pre-embedding immunocytochemistry for electron microscopy the antigen was observed on the luminal endothelial cell surface. CONCLUSION--Surprisingly, some non-barrier vessels in the ciliary body and choroid expressed EBA suggesting that it may play a broader role in endothelial properties than previously recognised. The functional significance of EBA remains to be elucidated.

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These references are in PubMed. This may not be the complete list of references from this article.

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