Abstract
BACKGROUND--The Surecell herpes (HSV) test kit is a test for detecting the presence of herpes simplex viral antigen by means of a monoclonal antibody based immunoassay. The test has proved to be highly sensitive and specific in diagnosing genital, oral, and dermatological herpes infections. METHODS--In this study, samples from patients with ocular keratitis were evaluated by tissue cultures and the Surecell test. The eyes of New Zealand rabbits were then inoculated with HSV type 1 acute keratitis, acute Staphylococcus keratitis, and HSV type 1 postkeratitis (healed corneas). Tear film samples collected from each eye with a cotton swab were evaluated by routine culture (A-549 monolayers) and by the Surecell test with and without prior placement of the swab in Hank's medium. RESULTS--The Surecell system had a 70% sensitivity and a 100% specificity in the detection of HSV antigen in ocular infections, and was shown to be a quick, efficient, and accurate method of testing for HSV antigen in humans. CONCLUSION--These results from humans and rabbits indicate that the Surecell test, which requires no special equipment, can be a useful in office adjunct in the clinical diagnosis of ocular herpes simplex.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cleveland P. H., Richman D. D., Redfield D. C., Disharoon D. R., Binder P. S., Oxman M. N. Enzyme immunofiltration technique for rapid diagnosis of herpes simplex virus eye infections in a rabbit model. J Clin Microbiol. 1982 Oct;16(4):676–685. doi: 10.1128/jcm.16.4.676-685.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coleman V. R., Thygeson P., Dawson C. R., Jawetz E. Isolation of virus from herpetic keratitis. Influence of idoxuridine on isolation rates. Arch Ophthalmol. 1969 Jan;81(1):22–24. doi: 10.1001/archopht.1969.00990010024004. [DOI] [PubMed] [Google Scholar]
- Denoyel G. A., Gaspar A., Nouyrigat C. Enzyme immunoassay for measurement of antibodies to herpes simplex virus infection: comparison with complement fixation, immunofluorescent-antibody, and neutralization techniques. J Clin Microbiol. 1980 Feb;11(2):114–119. doi: 10.1128/jcm.11.2.114-119.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunkel E. C., Pavan-Langston D., Fitzpatrick K., Cukor G. Rapid detection of herpes simplex virus (HSV) antigen in human ocular infections. Curr Eye Res. 1988 Jul;7(7):661–666. doi: 10.3109/02713688809033194. [DOI] [PubMed] [Google Scholar]
- Kowalski R. P., Gordon Y. J. Evaluation of immunologic tests for the detection of ocular herpes simplex virus. Ophthalmology. 1989 Nov;96(11):1583–1586. doi: 10.1016/s0161-6420(89)32683-2. [DOI] [PubMed] [Google Scholar]
- Lee S. M., Taguchi F. Rapid identification and typing of herpes simplex virus by a new enzyme immunoassay with peroxidase-labeled complement C1q. Arch Virol. 1989;108(3-4):247–257. doi: 10.1007/BF01310937. [DOI] [PubMed] [Google Scholar]
- Schwab I. R., Raju V. K., McClung J. Indirect immunofluorescent antibody diagnosis of herpes simplex with upper tarsal and corneal scrapings. Ophthalmology. 1986 Jun;93(6):752–756. doi: 10.1016/s0161-6420(86)33676-5. [DOI] [PubMed] [Google Scholar]
- Storch G. A., Reed C. A., Dalu Z. A. Evaluation of a latex agglutination test for herpes simplex virus. J Clin Microbiol. 1988 Apr;26(4):787–788. doi: 10.1128/jcm.26.4.787-788.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zaitseva N. S., Muravieva T. V., Vinogradova V. L., Shubladze A. K., Maevskaya T. M., Kasparov A. A., Rzhechitskaya O. V. Comparative evaluation of methods for laboratory diagnosis of herpetic eye disease. Am J Ophthalmol. 1973 Jun;75(6):997–1003. doi: 10.1016/0002-9394(73)91088-x. [DOI] [PubMed] [Google Scholar]