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. 1995 Jun;79(6):590–595. doi: 10.1136/bjo.79.6.590

Effect of TNF, IL-1, and IL-6 on the proliferation of human Tenon's capsule fibroblasts in tissue culture.

I A Cunliffe 1, P S Richardson 1, R C Rees 1, I G Rennie 1
PMCID: PMC505172  PMID: 7626577

Abstract

Trabeculectomy is a commonly performed procedure for primary open angle glaucoma and is successful in the majority of cases. However, certain factors including aphakia, previous surgery, secondary glaucomas, ethnic origin, and the long term use of topical antiglaucoma medications may be associated with a reduced success rate. The mechanism (or mechanisms) which influence clinical outcome following trabeculectomy remain elusive. Alterations in the composition of the conjunctiva or aqueous humour may be partly responsible for this effect, and this could be mediated by cytokines. In this study we found that tumour necrosis factor (TNF), and interleukin 1 (IL-1) were capable of stimulating the proliferation of Tenon's capsule fibroblasts in tissue culture. Interleukin 6 (IL-6) did not appear to have any effect. The relevance of this to wound healing following trabeculectomy is discussed.

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Selected References

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  1. Agelli M., Wahl S. M. Cytokines and fibrosis. Clin Exp Rheumatol. 1986 Oct-Dec;4(4):379–388. [PubMed] [Google Scholar]
  2. Allen R. C., Bellows A. R., Hutchinson B. T., Murphy S. D. Filtration surgery in the treatment of neovascular glaucoma. Ophthalmology. 1982 Oct;89(10):1181–1187. doi: 10.1016/s0161-6420(82)34672-2. [DOI] [PubMed] [Google Scholar]
  3. Batterbury M., Wishart P. K. Is high initial aqueous outflow of benefit in trabeculectomy? Eye (Lond) 1993;7(Pt 1):109–112. doi: 10.1038/eye.1993.23. [DOI] [PubMed] [Google Scholar]
  4. Beauchamp G. R., Parks M. M. Filtering surgery in children: barriers to success. Ophthalmology. 1979 Jan;86(1):170–180. doi: 10.1016/s0161-6420(79)35547-6. [DOI] [PubMed] [Google Scholar]
  5. Broadway D. C., Bates A. K., Lightman S. L., Grierson I., Hitchings R. A. The importance of cellular changes in the conjunctiva of patients with uveitic glaucoma undergoing trabeculectomy. Eye (Lond) 1993;7(Pt 4):495–501. doi: 10.1038/eye.1993.108. [DOI] [PubMed] [Google Scholar]
  6. Broadway D., Grierson I., Hitchings R. Racial differences in the results of glaucoma filtration surgery: are racial differences in the conjunctival cell profile important? Br J Ophthalmol. 1994 Jun;78(6):466–475. doi: 10.1136/bjo.78.6.466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. De Vos A. F., Hoekzema R., Kijlstra A. Cytokines and uveitis, a review. Curr Eye Res. 1992 Jun;11(6):581–597. doi: 10.3109/02713689209001814. [DOI] [PubMed] [Google Scholar]
  8. Dinarello C. A. Interleukin-1. Rev Infect Dis. 1984 Jan-Feb;6(1):51–95. doi: 10.1093/clinids/6.1.51. [DOI] [PubMed] [Google Scholar]
  9. Duncan M. R., Berman B. Differential regulation of collagen, glycosaminoglycan, fibronectin, and collagenase activity production in cultured human adult dermal fibroblasts by interleukin 1-alpha and beta and tumor necrosis factor-alpha and beta. J Invest Dermatol. 1989 May;92(5):699–706. doi: 10.1111/1523-1747.ep12696891. [DOI] [PubMed] [Google Scholar]
  10. Duncan M. R., Berman B. Stimulation of collagen and glycosaminoglycan production in cultured human adult dermal fibroblasts by recombinant human interleukin 6. J Invest Dermatol. 1991 Oct;97(4):686–692. doi: 10.1111/1523-1747.ep12483971. [DOI] [PubMed] [Google Scholar]
  11. Elias J. A., Freundlich B., Kern J. A., Rosenbloom J. Cytokine networks in the regulation of inflammation and fibrosis in the lung. Chest. 1990 Jun;97(6):1439–1445. doi: 10.1378/chest.97.6.1439. [DOI] [PubMed] [Google Scholar]
  12. Forster D. J., Rao N. A., Hill R. A., Nguyen Q. H., Baerveldt G. Incidence and management of glaucoma in Vogt-Koyanagi-Harada syndrome. Ophthalmology. 1993 May;100(5):613–618. doi: 10.1016/s0161-6420(93)31604-0. [DOI] [PubMed] [Google Scholar]
  13. Franks W. A., Limb G. A., Stanford M. R., Ogilvie J., Wolstencroft R. A., Chignell A. H., Dumonde D. C. Cytokines in human intraocular inflammation. Curr Eye Res. 1992;11 (Suppl):187–191. doi: 10.3109/02713689208999531. [DOI] [PubMed] [Google Scholar]
  14. Gillery P., Coustry F., Pujol J. P., Borel J. P. Inhibition of collagen synthesis by interleukin-1 in three-dimensional collagen lattice cultures of fibroblasts. Experientia. 1989 Jan 15;45(1):98–101. doi: 10.1007/BF01990461. [DOI] [PubMed] [Google Scholar]
  15. Goldring M. B., Goldring S. R. Cytokines and cell growth control. Crit Rev Eukaryot Gene Expr. 1991;1(4):301–326. [PubMed] [Google Scholar]
  16. Gressel M. G., Heuer D. K., Parrish R. K., 2nd Trabeculectomy in young patients. Ophthalmology. 1984 Oct;91(10):1242–1246. doi: 10.1016/s0161-6420(84)34179-3. [DOI] [PubMed] [Google Scholar]
  17. Gross R. L., Feldman R. M., Spaeth G. L., Steinmann W. C., Spiegel D., Katz L. J., Wilson R. P., Varma R., Moster M. R., Marks S. Surgical therapy of chronic glaucoma in aphakia and pseudophakia. Ophthalmology. 1988 Sep;95(9):1195–1201. doi: 10.1016/s0161-6420(88)33027-7. [DOI] [PubMed] [Google Scholar]
  18. Heuer D. K., Gressel M. G., Parrish R. K., 2nd, Anderson D. R., Hodapp E., Palmberg P. F. Trabeculectomy in aphakic eyes. Ophthalmology. 1984 Sep;91(9):1045–1051. doi: 10.1016/s0161-6420(84)34196-3. [DOI] [PubMed] [Google Scholar]
  19. Hugkulstone C. E., Smith L. F., Vernon S. A. Trabeculectomy in diabetic patients with glaucoma. Eye (Lond) 1993;7(Pt 4):502–506. doi: 10.1038/eye.1993.109. [DOI] [PubMed] [Google Scholar]
  20. Inaba Z. Long-term results of trabeculectomy in the Japanese: an analysis by life-table method. Jpn J Ophthalmol. 1982;26(4):361–373. [PubMed] [Google Scholar]
  21. Jampel H. D. Ascorbic acid is cytotoxic to dividing human Tenon's capsule fibroblasts. A possible contributing factor in glaucoma filtration surgery success. Arch Ophthalmol. 1990 Sep;108(9):1323–1325. doi: 10.1001/archopht.1990.01070110139038. [DOI] [PubMed] [Google Scholar]
  22. Jampel H. D., Roche N., Stark W. J., Roberts A. B. Transforming growth factor-beta in human aqueous humor. Curr Eye Res. 1990 Oct;9(10):963–969. doi: 10.3109/02713689009069932. [DOI] [PubMed] [Google Scholar]
  23. Jones N. P. Glaucoma in Fuchs' Heterochromic Uveitis: aetiology, management and outcome. Eye (Lond) 1991;5(Pt 6):662–667. doi: 10.1038/eye.1991.122. [DOI] [PubMed] [Google Scholar]
  24. Kanski J. J., Shun-Shin G. A. Systemic uveitis syndromes in childhood: an analysis of 340 cases. Ophthalmology. 1984 Oct;91(10):1247–1252. doi: 10.1016/s0161-6420(84)34177-x. [DOI] [PubMed] [Google Scholar]
  25. Katz L. J., Spaeth G. L. Surgical management of the secondary glaucomas: Part I. Ophthalmic Surg. 1987 Nov;18(11):826–834. [PubMed] [Google Scholar]
  26. Lavin M. J., Wormald R. P., Migdal C. S., Hitchings R. A. The influence of prior therapy on the success of trabeculectomy. Arch Ophthalmol. 1990 Nov;108(11):1543–1548. doi: 10.1001/archopht.1990.01070130045027. [DOI] [PubMed] [Google Scholar]
  27. Longstaff S., Wormald R. P., Mazover A., Hitchings R. A. Glaucoma triple procedures: efficacy of intraocular pressure control and visual outcome. Ophthalmic Surg. 1990 Nov;21(11):786–793. [PubMed] [Google Scholar]
  28. McMillan T. A., Stewart W. C., Hennis H. L., Hunt H. H., Apple D. J. Histologic differences in the conjunctiva of black and white glaucoma patients. Ophthalmic Surg. 1992 Nov;23(11):762–765. [PubMed] [Google Scholar]
  29. Merritt J. C. Filtering procedures in American blacks. Ophthalmic Surg. 1980 Feb;11(2):91–94. [PubMed] [Google Scholar]
  30. Miller M. H., Rice N. S. Trabeculectomy combined with beta irradiation for congenital glaucoma. Br J Ophthalmol. 1991 Oct;75(10):584–590. doi: 10.1136/bjo.75.10.584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Miller R. D., Barber J. C. Trabeculectomy in black patients. Ophthalmic Surg. 1981 Jan;12(1):46–50. [PubMed] [Google Scholar]
  32. Murray P. I., Hoekzema R., van Haren M. A., de Hon F. D., Kijlstra A. Aqueous humor interleukin-6 levels in uveitis. Invest Ophthalmol Vis Sci. 1990 May;31(5):917–920. [PubMed] [Google Scholar]
  33. O'Garra A. Interleukins and the immune system 1. Lancet. 1989 Apr 29;1(8644):943–947. [PubMed] [Google Scholar]
  34. Sato T., Ito A., Mori Y. Interleukin 6 enhances the production of tissue inhibitor of metalloproteinases (TIMP) but not that of matrix metalloproteinases by human fibroblasts. Biochem Biophys Res Commun. 1990 Jul 31;170(2):824–829. doi: 10.1016/0006-291x(90)92165-v. [DOI] [PubMed] [Google Scholar]
  35. Schmidt J. A., Mizel S. B., Cohen D., Green I. Interleukin 1, a potential regulator of fibroblast proliferation. J Immunol. 1982 May;128(5):2177–2182. [PubMed] [Google Scholar]
  36. Sherwood M. B., Grierson I., Millar L., Hitchings R. A. Long-term morphologic effects of antiglaucoma drugs on the conjunctiva and Tenon's capsule in glaucomatous patients. Ophthalmology. 1989 Mar;96(3):327–335. doi: 10.1016/s0161-6420(89)32888-0. [DOI] [PubMed] [Google Scholar]
  37. Stürmer J., Broadway D. C., Hitchings R. A. Young patient trabeculectomy. Assessment of risk factors for failure. Ophthalmology. 1993 Jun;100(6):928–939. doi: 10.1016/s0161-6420(93)31552-6. [DOI] [PubMed] [Google Scholar]
  38. Thornton S. C., Por S. B., Walsh B. J., Penny R., Breit S. N. Interaction of immune and connective tissue cells: I. The effect of lymphokines and monokines on fibroblast growth. J Leukoc Biol. 1990 Apr;47(4):312–320. doi: 10.1002/jlb.47.4.312. [DOI] [PubMed] [Google Scholar]
  39. Tomey K. F., Traverso C. E. The glaucomas in aphakia and pseudophakia. Surv Ophthalmol. 1991 Sep-Oct;36(2):79–112. doi: 10.1016/0039-6257(91)90124-x. [DOI] [PubMed] [Google Scholar]
  40. Vilcek J., Palombella V. J., Henriksen-DeStefano D., Swenson C., Feinman R., Hirai M., Tsujimoto M. Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors. J Exp Med. 1986 Mar 1;163(3):632–643. doi: 10.1084/jem.163.3.632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. de Vos A. F., van Haren M. A., Verhagen C., Hoekzema R., Kijlstra A. Kinetics of intraocular tumor necrosis factor and interleukin-6 in endotoxin-induced uveitis in the rat. Invest Ophthalmol Vis Sci. 1994 Mar;35(3):1100–1106. [PubMed] [Google Scholar]

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