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. 1992 Dec 1;89(23):11292–11296. doi: 10.1073/pnas.89.23.11292

Yeast RNA polymerase II initiation factor e: isolation and identification as the functional counterpart of human transcription factor IIB.

H Tschochner 1, M H Sayre 1, P M Flanagan 1, W J Feaver 1, R D Kornberg 1
PMCID: PMC50536  PMID: 1454810

Abstract

Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided in a single polypeptide of approximately 41 kDa, identified by peptide sequence analysis as the product of the SUA7 gene. Factor e interacted specifically with RNA polymerase II, consistent with a proposed role in determining the start site of transcription.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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