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. 2016 Apr 20;7(19):26979–26991. doi: 10.18632/oncotarget.8876

Figure 5. The membrane-associated Ccnd1 enhances lung metastatic activity of endometrial tumor cells.

Figure 5

A. MFE cells stably expressing luciferase were used for metastasis assay. Cells harboring Ccnd1 or Ccnd1-CAAX were injected in SCID-hairless mice. Four weeks after injection, animals expressing Ccnd1-CAAX showed lung metastasis. Representative images of the body luminescence (top) and lungs luminiscence (bottom) are shown. B. The number of lung nodules from eight animals in A was inferred from luminescence and represented. Significance values were determined by a Mann-Whitney test. C. Representative HA-immunohistochemistry images of lung nodules are shown. D. MFE cells expressed similar amounts of Ccnd1 or Ccnd1-CAAX. A protein extract was obtained from the cells before injection to the mice, and the amount of Ccnd1 was analyzed by immunoblot. Actin was used as a loading control.