Figure 1. PRL-3 renders INA-6 cells less dependent on IL-6.

Effect on ³H-thymidine incorporation (A) and survival as determined by annexin V-Alexa647 flow cytometry (B) of PRL-3 over-expression in human myeloma cell line INA-6, in the absence or the presence of suboptimal (10 pg/mL) or optimal (1 ng/mL) IL-6 concentrations. Error bars represent + 2 SD of quadruple (A) or duplicate (B) measurements. Figures show one representative experiment of three. Asterisk (*) denotes statistically significant difference from control (P < 0.05). PRL-3 overexpression in INA-6 cell line was confirmed by qRT-PCR (C) and by Western blotting (D).