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. 1996 Jan;80(1):74–77. doi: 10.1136/bjo.80.1.74

Collagen synthesis activity in the aqueous humour of eyes with glaucoma surgery: a pilot study.

A Tuulonen 1, J Risteli 1, L Risteli 1, J Välimäki 1, P J Airaksinen 1
PMCID: PMC505388  PMID: 8664238

Abstract

AIMS: The purpose of this pilot study was to test whether the rate of collagen synthesis is measurable in the aqueous humour samples in reoperated and previously unoperated eyes. METHODS: The material consisted of 28 eyes of 27 patients, aged 5 to 82 years, in whom aqueous humour samples were obtained during eye surgery. Fifteen patients had no history of previous eye surgery (control group) while 12 patients were re-operated (study group). The carboxyterminal propeptide of type I procollagen (PICP) and the aminoterminal propeptide of type III procollagen (PIIINP) were measured by specific immunoassays in the aqueous humour samples. RESULTS: The mean concentration of PIIINP in the study group (8.4 (SD 12.5) micrograms/l) was statistically significantly larger than that of the control group (0.4 (0.4) micrograms/l) (p < 0.0037). The respective values for PICP were 98.8 (SD 177.7) micrograms/l in the study group and 0.7 (SD 2.8) micrograms/l in the control group (p < 0.0005). The eyes in the study group which were re-operated within 1 year showed values increased 20-fold compared with the eyes in the control group and those eyes in the study group which had had their previous operation more than a year ago. In three eyes aqueous humour samples were also obtained from the encapsulated Molteno bleb and showed values increased 12-fold compared with those from the anterior chamber. CONCLUSIONS: PICP and PIIINP immunoassays are suitable for measuring the rate of collagen synthesis in the aqueous humour and may be useful in studies on pharmacological modulation of wound healing in glaucoma surgery.

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Selected References

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  1. Addicks E. M., Quigley H. A., Green W. R., Robin A. L. Histologic characteristics of filtering blebs in glaucomatous eyes. Arch Ophthalmol. 1983 May;101(5):795–798. doi: 10.1001/archopht.1983.01040010795021. [DOI] [PubMed] [Google Scholar]
  2. Blumenkranz M. S., Claflin A., Hajek A. S. Selection of therapeutic agents for intraocular proliferative disease. Cell culture evaluation. Arch Ophthalmol. 1984 Apr;102(4):598–604. doi: 10.1001/archopht.1984.01040030470029. [DOI] [PubMed] [Google Scholar]
  3. Giangiacomo J., Dueker D. K., Adelstein E. H. Histopathology of triamcinolone in the subconjunctiva. Ophthalmology. 1987 Feb;94(2):149–153. [PubMed] [Google Scholar]
  4. Giangiacomo J., Dueker D. K., Adelstein E. The effect of preoperative subconjunctival triamcinolone administration on glaucoma filtration. I. Trabeculectomy following subconjunctival triamcinolone. Arch Ophthalmol. 1986 Jun;104(6):838–841. doi: 10.1001/archopht.1986.01050180072032. [DOI] [PubMed] [Google Scholar]
  5. Gressel M. G., Heuer D. K., Parrish R. K., 2nd Trabeculectomy in young patients. Ophthalmology. 1984 Oct;91(10):1242–1246. doi: 10.1016/s0161-6420(84)34179-3. [DOI] [PubMed] [Google Scholar]
  6. Haukipuro K., Melkko J., Risteli L., Kairaluoma M., Risteli J. Synthesis of type I collagen in healing wounds in humans. Ann Surg. 1991 Jan;213(1):75–80. doi: 10.1097/00000658-199101000-00013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haukipuro K., Risteli L., Kairaluoma M. I., Risteli J. Aminoterminal propeptide of type III procollagen in healing wound in humans. Ann Surg. 1987 Dec;206(6):752–756. doi: 10.1097/00000658-198712000-00011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heuer D. K., Parrish R. K., 2nd, Gressel M. G., Hodapp E., Palmberg P. F., Anderson D. R. 5-fluorouracil and glaucoma filtering surgery. II. A pilot study. Ophthalmology. 1984 Apr;91(4):384–394. doi: 10.1016/s0161-6420(84)34291-9. [DOI] [PubMed] [Google Scholar]
  9. Hitchings R. A., Grierson I. Clinico pathological correlation in eyes with failed fistulizing surgery. Trans Ophthalmol Soc U K. 1983;103(Pt 1):84–88. [PubMed] [Google Scholar]
  10. Joseph J. P., Grierson I., Hitchings R. A. Chemotactic activity of aqueous humor. A cause of failure of trabeculectomies? Arch Ophthalmol. 1989 Jan;107(1):69–74. doi: 10.1001/archopht.1989.01070010071030. [DOI] [PubMed] [Google Scholar]
  11. KORNBLUETH W., TENENBAUM E. The inhibitory effect of aqueous humor on the growth of cells in tissue cultures. Am J Ophthalmol. 1956 Jul;42(1):70–74. doi: 10.1016/0002-9394(56)90011-3. [DOI] [PubMed] [Google Scholar]
  12. Melkko J., Niemi S., Risteli L., Risteli J. Radioimmunoassay of the carboxyterminal propeptide of human type I procollagen. Clin Chem. 1990 Jul;36(7):1328–1332. [PubMed] [Google Scholar]
  13. Molteno A. C. New implant for drainage in glaucoma. Clinical trial. Br J Ophthalmol. 1969 Sep;53(9):606–615. doi: 10.1136/bjo.53.9.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Radius R. L., Herschler J., Claflin A., Fiorentino G. Aqueous humor changes after experimental filtering surgery. Am J Ophthalmol. 1980 Feb;89(2):250–254. doi: 10.1016/0002-9394(80)90119-1. [DOI] [PubMed] [Google Scholar]
  15. Richter C. U., Shingleton B. J., Bellows A. R., Hutchinson B. T., O'Connor T., Brill I. The development of encapsulated filtering blebs. Ophthalmology. 1988 Sep;95(9):1163–1168. doi: 10.1016/s0161-6420(88)33041-1. [DOI] [PubMed] [Google Scholar]
  16. Risteli J., Niemi S., Trivedi P., Mäentausta O., Mowat A. P., Risteli L. Rapid equilibrium radioimmunoassay for the amino-terminal propeptide of human type III procollagen. Clin Chem. 1988 Apr;34(4):715–718. [PubMed] [Google Scholar]
  17. Roth S. M., Spaeth G. L., Starita R. J., Birbillis E. M., Steinmann W. C. The effects of postoperative corticosteroids on trabeculectomy and the clinical course of glaucoma: five-year follow-up study. Ophthalmic Surg. 1991 Dec;22(12):724–729. [PubMed] [Google Scholar]
  18. Sherwood M. B., Grierson I., Millar L., Hitchings R. A. Long-term morphologic effects of antiglaucoma drugs on the conjunctiva and Tenon's capsule in glaucomatous patients. Ophthalmology. 1989 Mar;96(3):327–335. doi: 10.1016/s0161-6420(89)32888-0. [DOI] [PubMed] [Google Scholar]
  19. Skuta G. L., Parrish R. K., 2nd Wound healing in glaucoma filtering surgery. Surv Ophthalmol. 1987 Nov-Dec;32(3):149–170. doi: 10.1016/0039-6257(87)90091-9. [DOI] [PubMed] [Google Scholar]
  20. Susanna R., Jr, Nicolela M. T., Takahashi W. Y. Mitomycin C as adjunctive therapy with glaucoma implant surgery. Ophthalmic Surg. 1994 Jul;25(7):458–462. [PubMed] [Google Scholar]

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