Skip to main content
NCBI home page
The British Journal of Ophthalmology logoLink to The British Journal of Ophthalmology
. 1996 May;80(5):475–479. doi: 10.1136/bjo.80.5.475

Measurement of retinal blood flow with fluorescein leucocyte angiography using a scanning laser ophthalmoscope in rabbits.

Y Yang 1, S Moon 1, S Lee 1, J Kim 1
PMCID: PMC505502  PMID: 8695572

Abstract

AIMS: To measure blood flow in the rabbit retinal circulation with fluorescein leucocyte angiography using a scanning laser ophthalmoscope. METHODS: Blood was withdrawn from the ear vein of a rabbit (New Zealand White), mixed with fluorescent dye in a test tube and centrifuged. The yellow-brown layer containing fluorescein stained leucocytes was collected and injected into the ear vein of the same rabbit while performing fluorescein angiography with a scanning laser ophthalmoscope. The image of retinal angiography displaying circulating fluorescent leucocytes was recorded on video tape. From each frame of the video tape, the consecutive positions of fluorescein stained leucocytes were digitised using an image analysis system and the velocity of blood flow was calculated. RESULTS: Fluorescent leucocytes were clearly visualised in the retinal arteries, capillaries, and veins which allowed measurement of blood flow. The mean capillary velocity was 0.69 (SD 0.21) mm/s. The mean velocities of leucocytes measured in different sized vessels were as follows: 5.83 (2.42) mm/s in arteries over 50 microns, 3.33 (0.62) mm/s in those 35-50 microns, and 2.42 (1.08) mm/s in arteries under 35 microns, 3.08 (1.56) mm/s in veins over 50 microns, 2.79 (1.49) mm/s in those 35-50 microns, and 1.21 (0.50) mm/s in veins under 35 microns. Blood flow pulsation occurs in arteries, arterioles, veins, and venules but not capillaries. CONCLUSION: Fluorescein leucocyte angiography can be used for simultaneous measurement of the blood flow in retinal arteries, veins, and capillaries.

Full text

PDF
475

Images in this article

476Image
on p.476
478Image
on p.478
478Image
on p.478

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fallon T. J., Chowiencyzk P., Kohner E. M. Measurement of retinal blood flow in diabetes by the blue-light entoptic phenomenon. Br J Ophthalmol. 1986 Jan;70(1):43–46. doi: 10.1136/bjo.70.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Green G. J., Feke G. T., Goger D. G., McMeel J. W. Clinical application of the laser Doppler technique for retinal blood flow studies. Arch Ophthalmol. 1983 Jun;101(6):971–974. doi: 10.1001/archopht.1983.01040010971026. [DOI] [PubMed] [Google Scholar]
  3. Jung F., Kiesewetter H., Körber N., Wolf S., Reim M., Müller G. Quantification of characteristic blood-flow parameters in the vessels of the retina with a picture analysis system for video-fluorescence angiograms: initial findings. Graefes Arch Clin Exp Ophthalmol. 1983;221(3):133–136. doi: 10.1007/BF02133853. [DOI] [PubMed] [Google Scholar]
  4. Khoobehi B., Niesman M. R., Peyman G. A., Oncel M. Repetitive, selective angiography of individual vessels of the retina. Retina. 1989;9(2):87–96. doi: 10.1097/00006982-198909020-00003. [DOI] [PubMed] [Google Scholar]
  5. Khoobehi B., Peyman G. A. Fluorescent vesicle system. A new technique for measuring blood flow in the retina. Ophthalmology. 1994 Oct;101(10):1716–1726. [PubMed] [Google Scholar]
  6. Khoobehi B., Peyman G. A., Vo K. Laser-triggered repetitive fluorescein angiography. Ophthalmology. 1992 Jan;99(1):72–79. doi: 10.1016/s0161-6420(92)32007-x. [DOI] [PubMed] [Google Scholar]
  7. Kimura H., Kiryu J., Nishiwaki H., Ogura Y. A new fluorescent imaging procedure in vivo for evaluation of the retinal microcirculation in rats. Curr Eye Res. 1995 Mar;14(3):223–228. doi: 10.3109/02713689509033518. [DOI] [PubMed] [Google Scholar]
  8. Koyama T., Matsuo N., Shimizu K., Mihara M., Tsuchida Y., Wolf S., Reim M. Retinal circulation times in quantitative fluorescein angiography. Graefes Arch Clin Exp Ophthalmol. 1990;228(5):442–446. doi: 10.1007/BF00927258. [DOI] [PubMed] [Google Scholar]
  9. Nishiwaki H., Ogura Y., Kimura H., Kiryu J., Honda Y. Quantitative evaluation of leukocyte dynamics in retinal microcirculation. Invest Ophthalmol Vis Sci. 1995 Jan;36(1):123–130. [PubMed] [Google Scholar]
  10. Ohnishi Y., Fujisawa K., Ishibashi T., Kojima H. Capillary blood flow velocity measurements in cystoid macular edema with the scanning laser ophthalmoscope. Am J Ophthalmol. 1994 Jan 15;117(1):24–29. doi: 10.1016/s0002-9394(14)73011-9. [DOI] [PubMed] [Google Scholar]
  11. Preussner P. R., Richard G., Darrelmann O., Weber J., Kreissig I. Quantitative measurement of retinal blood flow in human beings by application of digital image-processing methods to television fluorescein angiograms. Graefes Arch Clin Exp Ophthalmol. 1983;221(3):110–112. doi: 10.1007/BF02133848. [DOI] [PubMed] [Google Scholar]
  12. Riva C. E., Feke G. T., Ben-Sira I. Fluorescein dye-dilution technique and retinal circulation. Am J Physiol. 1978 Mar;234(3):H315–H322. doi: 10.1152/ajpheart.1978.234.3.H315. [DOI] [PubMed] [Google Scholar]
  13. Tanaka T., Muraoka K., Shimizu K. Fluorescein fundus angiography with scanning laser ophthalmoscope. Visibility of leukocytes and platelets in perifoveal capillaries. Ophthalmology. 1991 Dec;98(12):1824–1829. doi: 10.1016/s0161-6420(91)32043-8. [DOI] [PubMed] [Google Scholar]
  14. Wolf S., Arend O., Toonen H., Bertram B., Jung F., Reim M. Retinal capillary blood flow measurement with a scanning laser ophthalmoscope. Preliminary results. Ophthalmology. 1991 Jun;98(6):996–1000. doi: 10.1016/s0161-6420(91)32192-4. [DOI] [PubMed] [Google Scholar]
  15. Woon W. H., Fitzke F. W., Bird A. C., Marshall J. Confocal imaging of the fundus using a scanning laser ophthalmoscope. Br J Ophthalmol. 1992 Aug;76(8):470–474. doi: 10.1136/bjo.76.8.470. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The British Journal of Ophthalmology are provided here courtesy of BMJ Publishing Group

RESOURCES