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. Author manuscript; available in PMC: 2016 Oct 8.
Published in final edited form as: Biochemistry. 2016 Feb 1;55(6):970–979. doi: 10.1021/acs.biochem.5b01227

Figure 6.

Figure 6

HPLC chromatograms for c-di-GMP PDE activities of E169A Mn-TM0186: (A) as-isolated, (B) as-isolated and preincubated with 80 μM ferrous ammonium sulfate (+Fe2+), or (C) as-isolated and preincubated with 10 mM manganous chloride (+Mn2+).