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. 2016 Apr 20;7(21):30536–30549. doi: 10.18632/oncotarget.8867

Figure 8. MI-2 suppressed NLRP3 inflammasome complex assembly induced by ATP.

Figure 8

(A) THP-1 cells were treated with MI-2 (4 μM) for 1 h, following by 1 h incubation of 5 mM ATP. ASC oligomerization and redistribution was determined by immunoblot of ASC in cross-linked pellets (upper panels) and in cell lysates (lower panels). (B) THP-1 cells were treated with MI-2, following by 15 min ATP treatment. Cell lysates were immunoprecipitated with anti-ASC. (C) LPS-primed adherent bone marrow derived macrophages (BMDM) were treated with MI-2 (4 μM) for 1 h, followed by 5 mM ATP treatment for 15 min. The treated cells were analyzed by immunofluorescence cytochemistry (×100).