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. 2016 Apr 18;7(21):30990–31000. doi: 10.18632/oncotarget.8824

Figure 6. KLF4 promoted human osteosarcoma cell growth and migration by regulating CRYAB expression.

Figure 6

shRNA mediated knockdown CRYAB vector was transfected into the MG63 cells with or without overexpression of KLF4. a–c. Colony formation assay was used to measure the clonogenicity. d–f. The cells were injected subcutaneously into either side of the posterior flank of the same nude mouse (n =6 per group). The tumor size is shown in Figure 6d. The black bar represents 1 cm. The final tumor weight is shown in Figure 6e. The levels of KLF4 and CRYAB were detected by western blot (f). *P<0.05, **p<0.01 and ***p<0.001 vs Ctr. g–h. Matrigel migration assay was used to determine the migration viability of the cells. Migrated cells were plotted as the average number of cells per field of view. Results are representative of three independent experiments. *P<0.05, **p<0.01 and ***p<0.001 vs Ctr. i. ERK, MEK and their phosphorylation were detected by western blot assay. j. The immunostaining analysis of KLF4 and CRYAB protein expression from human osteosarcoma tissue microarray. High expression of KLF4 and CRYAB were shown by being stained as brown. k. The correlation between the expression of KLF4 and CRYAB in human osteosarcoma tissues from 40 patients was shown.