Table 1.

PCR genotyping and RT primers, and conditions.

DOI: http://dx.doi.org/10.7554/eLife.15461.013

Gene	Forward primer	Reverse primer	Size
TEAD-HA	5’-ATCCATGCTTGTTACCTTCAG-3’	5’-ACTACAAGGACGATGACAAG-3’	460 bp
Internal Control	5’-CAGCTCTACATCACCTGCCA-3’	5’-CACTGGGAAGAGACACTCAG-3’	520 bp
PCR conditions	Denature: 94°C for 30 s Anneal: 56°C for 30 s Extend: 72°C for 60 s (34 cycles)
Dystrophin (WT)	5’-GCGCGAAACTCATCAAATATGCGTGTTAGTGT-3’	5’-GATACGCTGCTTTAATGCCTTTAGTCACTCAGATAGTTGAAGCCATTTTG-3’	134 bp
Dystrophin (mdx)	5’-GCGCGAAACTCATCAAATATGCGTGTTAGTGT-3’	5’-CGGCCTGTCACTCAGATAGTTGAAGCCATTTTA-3’	117 bp
PCR conditions	Denature: 94°C for 20 s Anneal: 60°C for 20 s Extend: 72°C for 20 s (5 cycles) Denature: 94°C for 20 s Anneal: 64°C for 20 s Extend: 72°C for 20 s (23 cycles)
utrophin	5’-AGTATGGGGACCTTGAAGCC-3’	5’-CGAGCGTTTATCCATTTGGT-3’
cycloA	5’-ATTTCTTTTGACTTGCGGGC-3’	5’-AGACTTGAAGGGGAATG-3’
actin	5’-CCCTAAGGCCAACCGTGAA-3’	5’-CAGCCTGGATGGCTACGTACA-3’