Abstract
Bacillus solimangrovi GH 2-4T was isolated from mangrove soil and subjected to whole genome sequencing on HiSeq platform and annotated on RAST. The nucleotide sequence of this genome was deposited into DDBJ/EMBL/GenBank under the accession MJEH00000000.
Keywords: Whole genome sequencing, Bacillus solimangrovi, Mangrove soil
| Specifications | |
|---|---|
| Organism/cell line/tissue | Bacillus solimangrovi |
| Strain | GH 2-4T |
| Sequencer or array type | HiSeq (Illumina) |
| Data format | Analyzed |
| Experimental factors | Microbial strain |
| Experimental features | Whole genome analysis and gene annotation of GH 2-4T |
| Consent | N/A |
| Sample source location | Mangrove soil |
1. Direct link to deposited data
2. Experimental design, materials and methods
Mangroves are salt tolerant small plants that grow in coastal saline water. They play important roles as refuge areas for diverse organisms and their ecosystem is distinguished from others because of periodic tidal flooding [1]. Previously, our group isolated a novel species of the genus Bacillus for the first time from mangrove soil and assigned strain GH2-4T (= KCTC 33142T = JCM 18994T = DSM 27083T) as the type strain of Bacillus solimangrovi sp. nov. [1]. Genomic DNA of GH2-4T was extracted using bead-beating technique [2] and sequenced on HiSeq 2000 (Illumina). De novo genome assembly was performed using SPAdes and annotation was conducted on RAST [3]. SEED viewer was used for subsystem functional categorization of the predicted open reading frames (ORFs) and for visualization [4].
3. Data description
Whole genome sequencing has generated and assembled into 64 contigs and the estimated genome size is 3,844,007 base pairs. The N50 of the genome is 104,661 bp and the G + C content of 35.2%. The genome was predicted to contain 3672 coding sequence, 38 tRNAs and 3 rRNAs. These genes were annotated and classified into 427 subsystems (Fig. 1). Most of the annotated genes were involved in amino acids and derivatives (389), protein metabolism (249), cofactors, vitamins, prosthetic groups and pigments formations (246), carbohydrate metabolism (222) (Fig. 1).
Fig. 1.
Subsystems of Bacillus solimangrovi GH 2-4T based on SEED database.
4. Nucleotide accession number
This whole genome project has been deposited at DDBJ/EMBL/GenBank under accession MJEH00000000.
Conflict of interest
The authors clarified that this work and writing has no conflict of interest.
Acknowledgements
This work was supported by the Research Program for Agricultural Science & Technology Development (Project No. PJ010168) and was partially supported by grants from the National Research Foundation of Korea (NRF) (2015M3C9A4053394 & 2016M3A9B6901518), the KRIBB Research Initiative Program (KGM4541622) and by a grant from of the Korea Health Technology R&D Project (HI14C0368).
References
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