Figure 6.

Folate synthesis appears to be linked to histamine production in L. reuteri 6475. (A) Folate synthesis in wild‐type L. reuteri 6475 with FolC2 necessary for production of dihydrofolate and FolC responsible for addition of a polyglutamate tail to tetrahydrofolate. Folate synthesis contributes to histamine production and the anti‐inflammatory effect of L. reuteri. (B) Inhibition of folate synthesis in L. reuteri 6475::folC2 leads to reduced histamine production and loss of anti‐inflammatory activity. (C) Inhibition of folate polyglutamylation in L. reuteri 6475::folC does not impact histamine production and L. reuteri anti‐inflammatory activity is preserved. Hdc, histidine decarboxylase.