Abstract
NYC is a B lymphoma cell line derived from B/W mice. Upon fusion of NYC cells with a plasmacytoma, which itself produces no immunoglobulin, the resulting NYCH hybridoma cells are Mott cells; i.e., they contain large intracellular vesicles filled with immunoglobulin, the so-called Russell bodies. When NYCH.kappa, a variant of NYCH that had lost the ability to produce heavy chain, was transfected with a heavy-chain construct, this concentration of immunoglobulin in the intracellular vesicles occurred only when the transfected immunoglobulin heavy chain had the same variable region as NYC. Moreover, unlike conventional Mott cells, the hybrid cells secrete immunoglobulin at a normal rate.
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- Alanen A., Pira U., Lassila O., Roth J., Franklin R. M. Mott cells are plasma cells defective in immunoglobulin secretion. Eur J Immunol. 1985 Mar;15(3):235–242. doi: 10.1002/eji.1830150306. [DOI] [PubMed] [Google Scholar]
- Burrows P. D., Beck G. B., Wabl M. R. Expression of mu and gamma immunoglobulin heavy chains in different cells of a cloned mouse lymphoid line. Proc Natl Acad Sci U S A. 1981 Jan;78(1):564–568. doi: 10.1073/pnas.78.1.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grosschedl R., Weaver D., Baltimore D., Costantini F. Introduction of a mu immunoglobulin gene into the mouse germ line: specific expression in lymphoid cells and synthesis of functional antibody. Cell. 1984 Oct;38(3):647–658. doi: 10.1016/0092-8674(84)90259-9. [DOI] [PubMed] [Google Scholar]
- Jäck H. M., Beck-Engeser G., Lee G., Wofsy D., Wabl M. Tumorigenesis mediated by an antigen receptor. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8482–8486. doi: 10.1073/pnas.89.18.8482. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kellenberger E. Form determination of the heads of bacteriophages. Eur J Biochem. 1990 Jun 20;190(2):233–248. doi: 10.1111/j.1432-1033.1990.tb15568.x. [DOI] [PubMed] [Google Scholar]
- Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Posnett D. N., Mouradian J., Mangraviti D. J., Wolf D. J. Mott cells in a patient with a lymphoproliferative disorder. Differentiation of a clone of B lymphocytes into Mott cells. Am J Med. 1984 Jul;77(1):125–130. doi: 10.1016/0002-9343(84)90446-7. [DOI] [PubMed] [Google Scholar]
- Schweitzer P. A., Taylor S. E., Shultz L. D. Synthesis of abnormal immunoglobulins by hybridomas from autoimmune "viable motheaten" mutant mice. J Cell Biol. 1991 Jul;114(1):35–43. doi: 10.1083/jcb.114.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tarlinton D., Stall A. M., Herzenberg L. A. Repetitive usage of immunoglobulin VH and D gene segments in CD5+ Ly-1 B clones of (NZB x NZW)F1 mice. EMBO J. 1988 Dec 1;7(12):3705–3710. doi: 10.1002/j.1460-2075.1988.tb03253.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Valetti C., Grossi C. E., Milstein C., Sitia R. Russell bodies: a general response of secretory cells to synthesis of a mutant immunoglobulin which can neither exit from, nor be degraded in, the endoplasmic reticulum. J Cell Biol. 1991 Nov;115(4):983–994. doi: 10.1083/jcb.115.4.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss S., Burrows P. D., Meyer J., Wabl M. R. A Mott cell hybridoma. Eur J Immunol. 1984 Aug;14(8):744–748. doi: 10.1002/eji.1830140814. [DOI] [PubMed] [Google Scholar]
- Wofsy D., Chiang N. Y. Proliferation of Ly-1 B cells in autoimmune NZB and (NZB x NZW)F1 mice. Eur J Immunol. 1987 Jun;17(6):809–814. doi: 10.1002/eji.1830170612. [DOI] [PubMed] [Google Scholar]