Antimicrobial potential of green synthesized CeO₂ nanoparticles from Olea europaea leaf extract

Abstract

This article reports the green fabrication of cerium oxide nanoparticles (CeO₂ NPs) using Olea europaea leaf extract and their applications as effective antimicrobial agents. O. europaea leaf extract functions as a chelating agent for reduction of cerium nitrate. The resulting CeO₂ NPs exhibit pure single-face cubic structure, which is examined by X-ray diffraction, with a uniform spherical shape and a mean size 24 nm observed through scanning electron microscopy and transmission electron microscopy. Ultraviolet-visible spectroscopy confirms the characteristic absorption peak of CeO₂ NPs at 315 nm. Fourier transform infrared spectroscopy reflects stretching frequencies at 459 cm⁻¹, showing utilization of natural components for the production of NPs. Thermal gravimetric analysis predicts the successful capping of CeO₂ NPs by bioactive molecules present in the plant extract. The antimicrobial studies show significant zone of inhibition against bacterial and fungal strains. The higher activities shown by the green synthesized NPs than the plant extract lead to the conclusion that they can be effectively used in biomedical application. Furthermore, reduction of cerium salt by plant extract will reduce environmental impact over chemical synthesis.

Introduction

Green synthesis of metallic oxide nanoparticles (NPs) is an emerging field of nanoscience and technology. Synthesis methodology and surface modification play a critical role in the physical, chemical, electrical, and optical properties of nanomaterials. Cerium oxide (CeO₂) is a semiconductor with a band gap energy of 3.0–3.9 eV and large excitation energy. In bulk form, CeO₂ is a singular rare earth oxide and does not show any crystallographic modifications by temperature increase up to its melting point.^1–5 Its various applications such as acting as a catalyst; use in sensor, solid oxide fuel cells, and sunscreen cosmetics; and its O₂ storage capacity, role in CO₂ solar conversion, and antibacterial applications are due to its idiosyncratic ability to alter the oxidation states.^6–10

Ordinarily, CeO₂ NPs are fabricated by physical and chemical methods including hydrothermal,¹¹ flame spray pyrolysis,¹² thermal decomposition,¹³ aqueous precipitation,¹⁴ and coprecipitation technique.¹⁵ In contrast, green synthesis of metallic oxide NPs offers several advantages such as cost-effectiveness, large-scale commercial production, and pharmaceutical applications. The CeO₂ NPs are considered less toxic toward cell lines, when compared to TiO₂ and ZnO.¹⁶ Biosynthesis of CeO₂ NPs using plant extracts such as Gloriosa superba L. and Hibiscus sabdariffa has been reported¹ with promising results.

Olea europaea belongs to the family Oleaceae and is native to tropical and warm temperate regions of the world.¹⁷ Mediterranean region serves as the major cultivation area, accounting for about 98% of the world’s olive production.¹⁸ O. europaea exhibits antioxidant, anti-inflammatory, antiatherogenic, anticancer, antimicrobial, and antiviral activities, along with hypolipidemic and hypoglycemic potential.¹⁹ Bioactive compounds principally present in olive leaves are oleuropein, followed by hydroxytyrosol, the flavone-7-glucosides of luteolin and apigenin, and verbascoside.^20,21

This study reports green synthesis of CeO₂ NPs utilizing O. europaea leaf extract as an effective chelating agent without any acid or base treatment. The synthesized CeO₂ NPs are further characterized for their antibacterial and antifungal potential.

Experimental procedures

Collection and processing of plant material

The authenticated O. europaea leaves were procured from Barani Agricultural Research Institute, Chakwal, Pakistan. Fresh leaves were washed with distilled water and air-dried under shade at room temperature to avoid photodissociation of bioactive compounds. For extract preparation, 20 g of dried leaves was ground and suspended in 200 mL of distilled water. The mixture was placed in a shaking incubator at 50°C for 2 hours at 50 rpm. Thereafter, it was filtered using Whatman No 1 filter paper and the filtrate was stored at room temperature for further usage.

Fabrication of CeO₂ NPs by O. europaea leaf extract

For green synthesis of CeO₂ NPs, 8.68 g of Ce(NO₃)₃ ⋅ 6H₂O was added to 200 mL of O. europaea leaf extract and stirred on a magnetic hotplate at 50°C, 1,500 rpm for 2 hours. The blackish brown CeO₂ NPs were collected by centrifugation at 10,000 rpm (GR BioTek, Orpington, England) for 10 minutes. The NPs were washed repeatedly with deionized water and finally dried in hot air oven at 60°C for ~6 hours and further annealed in Gallenkamp furnace (Apeldoorn, the Netherlands) at 500°C for 2 hours. The yellow-colored NPs obtained were stored in an air-tight jar at room temperature. A schematic diagram for the fabrication of CeO₂ NPs using O. europaea leaf extract is shown in Figure 1.

Figure 1.

Schematic diagram of fabrication of CeO₂ NPs using Olea europaea leaf extract.

Abbreviations: BARI, Barani Agricultural Research Institute; NPs, nanoparticles; SEM, scanning electron microscopy; O. europaea, Olea europaea.

Characterization of CeO₂ NPs

Scanning electron microscopy (SEM)

The size and shape of synthesized CeO₂ NPs were studied using JEOL-JSM-6490LA SEM (JEOL, Tokyo, Japan) operating at 20 kV with a counting rate of 2,838 cps.

Transmission electron microscopy (TEM)

To further investigate the internal morphology, TEM analysis of green fabricated CeO₂ NPs was performed by TEM (model no JEOL-1010) operating at 80 kV.

X-ray diffraction (XRD; crystallographic structure)

To examine the crystallographic structure of green synthesized CeO₂ NPs, XRD analysis was carried out using X’Pert³ Powder (PANalytical) with nickel monochromator in the range of 2θ from 20° to 80° using Cu Kα radiation of wavelength 1.5406 Å. Operating voltage of 40 kV with 30 mA current was provided at room temperature. To calculate the theoretical size of CeO₂ NPs, Scherrer’s equation (D=0.9λ/β cosθ) was employed, where D is the average crystalline domain size perpendicular to the reflecting planes, λ the X-ray wavelength (1.5406 Å), β the angular full width at half maximum in radians, and θ is the diffraction angle (2θ is the measured angle of diffraction in degrees) or Bragg’s angle. Furthermore, the lattice constant was measured using the following equation:

$$d=\frac{a}{\sqrt{h^2+k^2+I^2}}$$

where a=b=c are lattice constants, d represents the interplanner spacing which was calculated by using 2d sinθ = nλ, and h, k, and I are Miller indices. The unit cell volume was calculated using V=a³ formula unit.

Fourier transform infrared (FTIR) spectroscopy

For the identification of unknown bio-active compounds in plant extract, utilization of capping agents from plant extract and confirmation of production materials (CeO₂ NPs), FTIR spectroscopic analysis was performed using KBr pellet methodology (model SHIMADZU FTIR, Kyoto, Japan) in the wavenumber ranges 400–4,000 cm⁻¹. FTIR spectra for CeO₂ NPs, residue (as synthesized), and O. europaea leaf extract were recorded separately and explained comparatively.

Ultraviolet (UV)-visible spectroscopy

The optical characterization of green fabricated CeO₂ NPs was made through UV-visible spectroscopy in the range of 290–400 nm with a Shimadzu spectrophotometer (model UV-1800, Kyoto, Japan) operating at a resolution of 1 nm. Three milligrams of synthesized CeO₂ NPs was dissolved in 10 mL of deionized H₂O and this solution was sonicated for 20 minutes. Subsequently, the liquid sample was subjected to UV-visible spectrophotometry.

Thermal gravimetric analysis (TGA)

To examine the thermal properties and capping action of bioactive compounds for tailoring CeO₂ NPs, we performed TGA (model Diamond TGA; PerkinElmer, Waltham, USA) under nitrogen environment from 25°C to 800°C at 10°C/minute.

Antibacterial activity of CeO₂ NPs

The antibacterial activities of green synthesized CeO₂ NPs were studied against Gram-positive (G+ve) (Staphylococcus aureus ATCC 6538) and Gram-negative (G−ve) (Escherichia coli ATCC 15224, Pseudomonas aeruginosa ATCC 15442, Klebsiella pneumoniae ATCC-BAA 1706) strains by disk diffusion method with slight modification.¹ In brief, the bacterial strains were cultured in nutrient broth (Sigma-Aldrich Co., St Louis, MO, USA) at 37°C until the culture reached 1.5×10⁸ colony forming units (CFU) per milliliter. About 20 mL of autoclaved molten nutrient agar was poured into the Petri dishes and allowed to cool. All of the four bacterial cultures were swapped over solidified agar medium. Disks were loaded with 20 µg/5 µL CeO₂ NPs solution. A solution of cefixime or roxithromycin (4 mg/mL each) was used as positive control and deionized H₂O as negative control. The plates were incubated at 37°C for 24 hours and the zones of inhibition (ZOIs) around the disks were measured thereafter.

Antifungal assay

Antifungal activity was determined against Mucor species (FCBP-0300), Aspergillus flavus (FCBP-0064), Fusarium solani (FCBP-434), and Aspergillus niger (FCBP-0198) by disk diffusion method. Sabouraud dextrose agar (pH 5.7) (Sigma-Aldrich Co.) was autoclaved and poured in Petri plates ensuring sterile conditions. Fungal lawns were prepared by inoculating spores on the surface of the growth media. Thereafter, disks were loaded with CeO₂ NPs solution 20 µg/5 µL through micropipette. Clotrimazole 10 µg/disk was used as positive control, while deionized water served as negative control. The plates were incubated at 25°C for 24–48 hours and ZOI was measured.

CFU counting assay

To examine the percentage reduction in bacterial count with time, we carried out CFU counting assay. The experimentation was performed in sample tubes, each having 2 mL of sterilized (autoclaved) nutrient broth. The first sample tube containing 2 mL of nutrient broth acted as sterile negative control and the second tube was impregnated with cefixime (as positive control), while the remaining four samples were inoculated with S. aureus (G+ve), and E. coli, P. aeruginosa, and K. pneumoniae (G−ve), respectively, having a concentration of 10⁷ and 10⁹ CFU/mL each, and then placed in a shaking incubator at 37°C. Thereafter, the bacterial culture tubes were loaded with CeO₂ NPs solution (20 µg/5 µL). After an interval of 30 minutes, 10 µL from each sample was collected and spread over the already prepared agar plate surface. To estimate the bacterial colony in liquid, McFarland turbidity standards were applied, and their values were calibrated before and after incubation. Positive and negative control tests were performed on E. coli only. Later on, the plates were incubated at 37°C and the percentage reduction with time in bacterial count was calculated by the following approximation:²²

$$\%\text{reduction}=\frac{\text{Viable count at}0\text{minutes}-\text{Viable count at}150\text{minutes}}{\text{Viable count at}0\text{minutes}}\times 100$$

Statistical analysis

Results are calculated as the mean of at least three individual experiments presented with standard deviation (SD). The statistical analysis of the results was carried out with Student’s t-tests by using Statistical Package for the Social Sciences software package version 19.0 (IBM Corporation, Armonk, NY, USA) and considered significant at P-values <0.05.

Results and discussion

Biosynthesis process using O. europaea leaf extract (visual observations)

Synthesis of NPs using plant extracts has several advantages over other conventional biosynthesized routes (based on fungi, bacteria, etc). It is because the green extract is more ecofriendly and environment friendly, and fewer cytotoxic reducing agents are required for tailoring NPs. It is also commonly observed that maintaining microbial cultures under optimum conditions is time consuming and costly. In bulk (at an industrial level), safe mode synthesis of NPs, microbial-based NPs production has its own limitations.^23,24

O. europaea aqueous extract contains a variety of molecules that might be effectively utilized as chelating agents in the green synthesis of CeO₂ NPs. Upon addition of (CeNO₃)₃ 6H₂O, the biologically active compounds of the plant extract²¹ act as reducing agents for the nanofabrication process. Oleuropein is the most abundant bioactive compound in O. europaea aqueous extract, which breaks down into highly polarizable and reactive hydroxytyrosol.²¹ It may take part in the redox mechanism in tuning CeO₂ NPs, as shown in Figure 2. Figure 3 shows the change in color from dark blackish green to dirty green, indicating the formation of CeO₂ NPs. The blackish brown CeO₂ NPs were collected by centrifugation at 10,000 rpm for about 10 minutes. Thereafter, the NPs were washed repeatedly with deionized water to eliminate the uncoordinated biomolecules from the extract, and finally dried in hot air oven at 60°C for ~6 hours and further annealed at 500°C for 2 hours. The yellow-colored NPs obtained were stored at room temperature. The entire recovery process is presented in Figures 1 and 2.

Figure 2.

Hypothesized redox process of CeO₂ NPs synthesis.

Abbreviation: NPs, nanoparticles.

Figure 3.

Biosynthesis of CeO₂ NPs.

Abbreviations: NPs, nanoparticles; O. europaea, Olea europaea.

SEM analysis

Figure 4 depicts the SEM images of green synthesized CeO₂ NPs. It is obvious from the SEM micrograph that the CeO₂ NPs are highly homogeneous and symmetrical in morphology with spherical shape, and the findings clearly represent a mean size of 24 nm. Similar morphological shape with uniform average grain size of less than 30 nm was also reported previously.^10,25,26

Figure 4.

SEM images of green synthesized CeO₂ NPs. (A) Magnification ×30,000; (B) Magnification ×50,000.

Abbreviations: NPs, nanoparticles; SEM, scanning electron microscopy; SEI, scanning electron-microscopy instrument; NUST, National University of Sciences and Technology.

TEM analysis

It is clear from the TEM micrograph that the green synthesized CeO₂ NPs exhibit spherical morphology with highly homogeneous symmetry and an average particle size of 24 nm (Figure 5).

Figure 5.

TEM micrographs of green synthesized CeO₂ NPs.

Abbreviations: NPs, nanoparticles; TEM, transmission electron microscopy.

XRD (crystallographic structure) analysis

Figure 6 shows the XRD profile of green fabricated CeO₂ NPs. It shows that the CeO₂ NPs possess single-phase cubic fluorite structure without any additional peaks. Presence of several broad Bragg peaks corresponds to (111), (200), (220), (311), (222), (400), (331), and (420) orientations and they are precisely well indexed to Joint Committee on Powder Diffraction Standards (JCPDS) (card no 34-0394). This later crystallographic phase reflects that each cerium site is encountered by eight oxygen sites in face-centered cubic arrangement, while each oxygen has the geometry of tetrahedron cerium site. Using Debye–Scherrer approximation and from the broadening of peaks, the approximate crystal-lite size of CeO₂ NPs was found to be 6 nm, which shows that each grain of size 24 nm was composed of a cluster of 6 nm crystallites. The observed value was found to be close to that reported previously.²⁵ The calculated value of lattice constant “a” is 0.585 nm, while the unit cell volume “V” is measured to be 0.20 nm.

Figure 6.

Typical XRD analysis of CeO₂ NPs at room temperature.

Abbreviations: NPs, nanoparticles; XRD, X-ray diffraction.

FTIR spectroscopy analysis

Comparative FTIR results illustrate significant absorption peaks in the wave number range 400–4,000 cm⁻¹ (Figure 7). The range between 2,500 and near 4,000 cm⁻¹ is assigned to O–H and C–H stretch. The plant extract exhibiting N–O symmetric stretch at 1,355 cm⁻¹ was found to be less in the residue after CeO₂ NPs were isolated, reflecting that Ce is actively oxidized to CeO₂ by the nitro compounds in extract chemistry. The onset of a broad intense band that can be viewed as one tends toward higher vibrations (2,800 cm⁻¹) can be ascribed to O–H stretching range due to the presence of surface-adsorbed water molecules from the surroundings.²⁷ The spectra due to stretching frequency of Ce–O can be seen at 459 cm⁻¹, which indicates the fabrication of CeO₂ NPs. Similarly, Ce–O stretching band at 451 cm⁻¹ and at 450 cm⁻¹ was reported by Arumugam et al¹⁰ and Goharshadi et al,²⁸ respectively.

Figure 7.

FTIR spectra of biosynthesized CeO₂ NPs from Olea europaea leaf extract.

Notes: (a) Plant extract; (b) residue (as synthesized); (c) CeO₂ NPs.

Abbreviations: FTIR, Fourier transform infrared; NPs, nanoparticles.

UV-visible spectroscopy studies

Figure 8 illustrates the UV-visible absorption spectra of CeO₂ NPs, which demonstrates the presence of a single intensified peak at 315 nm that was also closely observed in previous studies.²⁵ The position of the absorption peak confirms that green synthesized CeO₂ NPs possess fluorite cubic morphology,²⁶ as justified in Figures 4 and 5.

Figure 8.

UV-Vis spectra of CeO₂ NPs.

Abbreviations: NPs, nanoparticles; UV-Vis, ultraviolet-visible spectroscopy.

TGA studies

TGA of biosynthesized CeO₂ NPs was performed to study the capping action of biomolecules and the thermal behavior of the synthesized CeO₂ NPs, which is shown in Figure 9. TGA represents different stages of decomposition. Weight loss occurring in the first step at temperatures from 25°C to 115°C was ascribed to the loss of surface water molecules.²⁹ The minor weight loss that occurred in the second step at temperatures from 50°C to 200°C was due to the combustion of bioactive surface organic components acting as capping agents. The slight weight loss occurring at around 600°C was because of oxygen loss at high temperature.³⁰ Our experimental observations show total weight loss of 0.52 mg (8.8%) of the sample.

Figure 9.

TGA curve of green synthesized CeO₂ NPs.

Abbreviations: NPs, nanoparticles; TGA, thermal gravimetric analysis.

Antibacterial analysis of CeO₂ NPs

The antibacterial assay was performed against G+ve and G−ve bacterial entities using CeO₂ NPs sample loaded at a concentration of 20 µg/05 µL on disks. Figure 10 reflects the measurements in size of ZOI around CeO₂ NPs poured disks. In the case of K. pneumoniae, S. aureus, and P. aeruginosa, a ZOI of 9, 10, and 8 mm was observed, respectively, which shows mild to moderate antibacterial behavior. Promising ZOI of 19 mm was recorded against E. coli; the prominent difference in ZOI might be due to the unique cell membrane structure that helps the bacterium to resist antimicrobial agents. Besides all these, other features such as the rate at which the NPs diffuse also play an important role against various bacterial strains.³¹ Figure 10 shows that G+ve bacteria are more susceptible to CeO₂ NPs than G−ve bacteria; similar trends were reported before.¹⁰ Green synthesized NPs have proven efficiency and comparatively low genotoxic and cytotoxic behavior toward healthy cells, when compared to NPs synthesized by various chemical methods.³² CeO₂ NPs fabricated by way of chemical methods were also found to be cytotoxic to somatic cells.²⁵ O. europaea leaf extract also takes part in inhibiting K. pneumoniae, S. aureus, and P. aeruginosa, with ZOI of 8, 7.6, and 7.8 mm, respectively; its potential activity has also been reported in previous studies.^33,34 Bioactive compounds from the leaf extract, such as oleuropein, are also found to be magically active in inhibiting several bacterial pathogens.³⁴

Figure 10.

Antibacterial analysis of CeO₂ NPs, plant extract, cefixime/roxithromycin (positive control), and deionized water (negative control), taking their mean values with ±SD.

Abbreviations: NPs, nanoparticles; SD, standard deviation.

From the results of XRD, SEM, and TEM, the average CeO₂ NP size was found to be 24 nm, with the smaller crystallite size being 6 nm. Higher surface area due to smaller crystal size results in greater antibacterial activity.³⁵ The antibacterial activity of CeO₂ NPs primarily depends upon the electrostatic attraction between positively charged NPs and negatively charged bacterial cell surface and is crucial for the activity of NPs as a bactericidal agent. The interaction is also responsible for reactive oxygen species (ROS) generation, which ultimately leads to bactericidal effect.^36,37 ROS include the highly reactive hydroxyl radical (OH), singlet oxygen (₁O²), and the least toxic superoxide anion radical (⁻*O₂), contributing to the major oxidative stress in biological systems.³⁸ ROS production is closely related to the efficiency of a photocatalyst, depending on the generation rate, rate of migration, and energy levels of the photoexcited electron–hole pairs. In the present study, such an increase in ROS levels is perhaps linked to the electronic and microstructure properties like grain size, specific surface area, pore size, and so on. The mechanism of photoexcited generation of ROS can be given as follows:¹⁰

$${\text{CeO}}_2+\mathrm{h}\upsilon \to {\mathrm{e}}^{-}+{\mathrm{h}}^{+}{\mathrm{h}}^{+}+{\mathrm{H}}_2\mathrm{O}\to ·\text{OH}+\mathrm{H}+{\mathrm{e}}^{-}+{\mathrm{O}}_2\to {{\mathrm{O}}_2}^{-}{\mathrm{O}}_2+{\mathrm{H}}^{+}\to {\text{HO}}_2·{\text{HO}}_2+{\mathrm{H}}^{+}+{\mathrm{e}}^{-}\to {\mathrm{H}}_2{\mathrm{O}}_2$$

Mostly, it is believed that NPs release ions which interact with the thiol (−SH) group of the protein residing at the bacterial cell surface,³⁹ resulting in denaturation of surface protein along with loss of cell membrane permeability, ultimately causing cell death. The rough surface for metallic NPs also intensifies their mechanical bactericidal capabilities of E. coli.⁴⁰ In our experimentation, UV analysis showed strong, intensified CeO₂ NPs absorption peaks at 315 nm. It is a well-known fact that Ce, as compared to its bulk, forms low band gap energy which results in its photoexcitation at room temperature.²⁵ This phenomenon will also assist in mass production of ROS inside bacterial cellular environment, and intoleration of ROS due to CeO₂ NPs results in malfunctioning of microbial biosynthetic machinery, causing lethality. In Figure 11 is presented a possible schematic diagram summarizing the above fact.

Figure 11.

Schematic illustration of CeO₂ NPs antibacterial activity.

Abbreviations: NPs, nanoparticles; ROS, reactive oxygen species.

Antifungal assay analysis of CeO₂ NPs

The antifungal properties of green synthesized CeO₂ NPs have been shown in Figure 12. A. flavus and A. niger show greater CeO₂ NPs susceptibility with ZOI of 19 mm than F. solani for which the ZOI is 10 mm. The fungicidal activity of CeO₂ NPs was found to be maximum in the case of Mucor species with ZOI of 22 mm, showing great competency as an antifungal agent, like other antifungal candidates.⁴¹ The exact mechanism of the antifungal activity of CeO₂ NPs is not understood, but the antimicrobial potential is probably due to the electromagnetic interaction and ROS generation,³⁶ like the production of lethal hydroxyl radical (OH) when present in the immediate vicinity of the lipid membrane. ROS cause oxidative deterioration of cell membrane lipids,⁴² denaturing the cell membrane permeability with leakage of potassium ions, ultimately causing cell death.³⁹

Figure 12.

Antifungal analysis of CeO₂ NPs, plant extract, clotrimazole (positive control), and deionized water (negative control), taking their mean values with ±SD.

Abbreviations: NPs, nanoparticles; SD, standard deviation.

Analysis of CFU counting assay

The results of progression in bacterial growth with time are documented in Figure 13. It is obvious that for all samples, the bacterial population was in lag phase during the first 20 minutes of the experiment, but with the addition of CeO₂ NPs (20 µg/5 µL), persistent decline in growth was observed. Reduction in viability of E. coli was markedly maximum, while for all other bacterial strains, the growth inhibition rate with time was almost similar. So, it is very clear that due to extremely small particle size and pure phase crystallinity with high surface area (Figures 4 and 5), CeO₂ NPs exhibit prominent bacteriostatic and bactericidal activity. This might be due to intolerance of metallic CeO₂ NPs by bacterial metabolic machinery, lethal ROS generation, and greater cytotoxicity toward microbial pathogens.^36,37 Future work regarding the molecular level interaction of CeO₂ NPs in bacterial growth inhibition needs more exploration.

Figure 13.

CFU counting assay of green synthesized CeO₂ NPs against G+ve and G−ve bacterial strains.

Abbreviations: CFU, colony forming units; G+ve, Gram positive; G−ve, Gram negative; NPs, nanoparticles.

Conclusion

The leaf extract of O. europaea is proved to be an effective chelating agent for the green synthesis of highly homogeneous, ecofriendly, and extremely small sized CeO₂ NPs. Both XRD and SEM findings confirm the cubic fluorite structure of CeO₂ NPs without any impurities and with a mean crystallite size of 6 nm and an average grain size of 24 nm. FTIR results reveal discharge of bioactive compounds from the leaf extract and Ce–O stretching mode at 459 cm⁻¹. This study shows that CeO₂ NPs exhibit mild to moderate antibacterial activity against both G+ve and G−ve strains, especially high susceptibility was shown by E. coli. CeO₂ NPs exhibited promising inhibitory effect against Mucor species, ensuring their antifungal properties. It can be deduced from this study that CeO₂ NPs synthesized through green chemistry have great potential for future antimicrobial therapies. The follow-up study will focus on differential cytotoxic behavior of photosynthesized CeO₂ NPs to healthy and cancer cells; more specifically, the size-dependent anticancer and antimicrobial properties will be examined.