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. 1992 Dec 15;89(24):11769–11773. doi: 10.1073/pnas.89.24.11769

Amplifiable messenger RNA.

Y Wu 1, D Y Zhang 1, F R Kramer 1
PMCID: PMC50638  PMID: 1465396

Abstract

RNA molecules were prepared that consisted of an mRNA encoding chloramphenicol acetyltransferase embedded within the sequence of midivariant RNA, which is a template for the RNA-directed RNA polymerase Q beta replicase. These recombinant RNAs were shown to be bifunctional: they are amplified exponentially by incubation with Q beta replicase, and the replicated RNA serves as template for the cell-free synthesis of enzymatically active chloramphenicol acetyltransferase. The availability of amplifiable mRNAs will enable relatively large amounts of protein to be synthesized in vitro.

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