Table 2. Temperature experiment.
Average quantities of Extracted DNA (ng), DNA extraction yield (ng DNA/mg tissue digested, see text for method of calculation) and Quality (% DNA > 9,416 bp) of genomic DNA extracted from tissues of two species fish (Morone americana) and crab (Callinectes sapidus) held in one of 25 treatments: five storage temperatures (Room Temperature = RT, 4, −20, −80, and −190 °C) × five preservative methods (95% EtOH, DMSO-EDTA, DNAzol, RNAlater, M2) for a minimum of 14 days. Mean and standard deviation (SD) values are shown.
| RT | 4 °C | −20 °C | −80 °C | −190 °C | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Extracted DNA (ng) | Extraction yield (ng DNA/mg tissue) | Quality (% > 9,416 bp) | Extracted DNA (ng) | Extraction yield (ng DNA/mg tissue) | Quality (% > 9,416 bp) | Extracted DNA (ng) | Extraction yield (ng DNA/mg tissue) | Quality (% > 9,416 bp) | Extracted DNA (ng) | Extraction yield (ng DNA/mg tissue) | Quality (% > 9,416 bp) | Extracted DNA (ng) | Extraction yield (ng DNA/mg tissue) | Quality (% > 9,416 bp) | ||||||||||||||||
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | |
| Fish: Morone americana | ||||||||||||||||||||||||||||||
| EtOH | 233 | 129 | 150 | 69 | 46 | 18 | 229 | 87 | 157 | 54 | 23 | 12 | 188 | 38 | 120 | 23 | 41 | 11 | 159 | 31 | 105 | 17 | 49 | 24 | 306 | 105 | 209 | 71 | 32 | 17 |
| DMSO | 375 | 170 | 248 | 96 | 89 | 10 | 295 | 116 | 196 | 66 | 89 | 8 | 309 | 155 | 208 | 108 | 94 | 6 | 372 | 192 | 263 | 132 | 95 | 13 | 488 | 244 | 326 | 189 | 82 | 20 |
| DNAzol | 298 | 177 | 194 | 109 | 98 | 4 | 297 | 114 | 192 | 74 | 83 | 15 | 294 | 143 | 202 | 90 | 96 | 6 | 447 | 184 | 307 | 127 | 100 | 0 | 609 | 238 | 403 | 157 | 40 | 42 |
| RNAlater | 173 | 127 | 125 | 102 | 100 | 0 | 169 | 44 | 109 | 23 | 59 | 14 | 160 | 28 | 111 | 13 | 81 | 6 | 184 | 78 | 125 | 57 | 77 | 10 | 174 | 61 | 117 | 35 | 71 | 23 |
| M2 | 161 | 73 | 110 | 52 | 82 | 4 | 180 | 33 | 134 | 18 | 30 | 17 | 188 | 87 | 142 | 64 | 80 | 10 | 164 | 45 | 116 | 31 | 88 | 6 | 269 | 66 | 172 | 33 | 63 | 13 |
| Crab: Callinectes sapidus | ||||||||||||||||||||||||||||||
| EtOH | 1,238 | 354 | 430 | 112 | 52 | 35 | 889 | 190 | 329 | 81 | 62 | 24 | 804 | 432 | 291 | 159 | 39 | 14 | 1,264 | 580 | 415 | 173 | 24 | 4 | 942 | 222 | 320 | 87 | 77 | 24 |
| DMSO | 888 | 367 | 320 | 155 | 79 | 6 | 388 | 162 | 141 | 54 | 37 | 20 | 727 | 345 | 257 | 98 | 45 | 27 | 864 | 472 | 308 | 186 | 24 | 3 | 715 | 284 | 241 | 101 | 49 | 28 |
| DNAzol | 643 | 252 | 242 | 100 | 90 | 10 | 705 | 436 | 257 | 177 | 77 | 27 | 511 | 371 | 171 | 110 | NA | NA | 920 | 270 | 332 | 110 | 28 | 3 | 432 | 213 | 151 | 72 | 91 | 12 |
| RNAlater | 641 | 175 | 229 | 56 | 62 | 23 | 704 | 242 | 271 | 108 | 55 | 11 | 399 | 131 | 142 | 50 | 54 | 7 | 772 | 667 | 289 | 271 | 36 | 16 | 532 | 320 | 178 | 90 | 65 | 24 |
| M2 | 582 | 166 | 222 | 67 | 84 | 15 | 606 | 282 | 227 | 105 | 73 | 21 | 750 | 194 | 268 | 81 | 59 | 19 | 848 | 474 | 309 | 171 | 78 | 23 | 1,024 | 273 | 336 | 93 | 60 | 28 |