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. 1992 Dec 15;89(24):12028–12032. doi: 10.1073/pnas.89.24.12028

Wild-type p53 binds to the TATA-binding protein and represses transcription.

E Seto 1, A Usheva 1, G P Zambetti 1, J Momand 1, N Horikoshi 1, R Weinmann 1, A J Levine 1, T Shenk 1
PMCID: PMC50691  PMID: 1465435

Abstract

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

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