Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1992 Dec 15;89(24):12078–12082. doi: 10.1073/pnas.89.24.12078

Organization of the human skeletal myosin heavy chain gene cluster.

S J Yoon 1, S H Seiler 1, R Kucherlapati 1, L Leinwand 1
PMCID: PMC50701  PMID: 1465443

Abstract

Myosin is an important structural and enzymatic component of skeletal muscle. Multiple myosin isoforms are encoded by a multigene family and are expressed in different developmental stages and fiber types. In humans and mice, skeletal myosin heavy chain (MYH) genes are clustered on a single chromosome (17p and 11, respectively). Since the structural organization of the gene cluster may affect its expression as well as shed light on MYH genetic alterations, a physical map of the human MYH gene cluster was constructed. Nine yeast artificial chromosomes containing MYH genes were isolated and used to construct a contiguous set (contig) of overlapping yeast artificial chromosomes. This contig encompasses a genetic marker mapped to 17p13.1. Six MYH genes were located within a 500-kilobase segment of human DNA. The order of the genes within this cluster does not correspond to the developmental pattern of expression of individual members.

Full text

PDF
12078

Images in this article

12080Image
on p.12080
12081Image
on p.12081

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burke D. T., Carle G. F., Olson M. V. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806–812. doi: 10.1126/science.3033825. [DOI] [PubMed] [Google Scholar]
  2. Bárány M. ATPase activity of myosin correlated with speed of muscle shortening. J Gen Physiol. 1967 Jul;50(6 Suppl):197–218. doi: 10.1085/jgp.50.6.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chu G., Vollrath D., Davis R. W. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582–1585. doi: 10.1126/science.3538420. [DOI] [PubMed] [Google Scholar]
  4. Cox R. D., Weydert A., Barlow D., Buckingham M. E. Three linked myosin heavy chain genes clustered within 370 kb of each other show independent transcriptional and post-transcriptional regulation during differentiation of a mouse muscle cell line. Dev Biol. 1991 Jan;143(1):36–43. doi: 10.1016/0012-1606(91)90052-5. [DOI] [PubMed] [Google Scholar]
  5. Davis R. W., Thomas M., Cameron J., St John T. P., Scherer S., Padgett R. A. Rapid DNA isolations for enzymatic and hybridization analysis. Methods Enzymol. 1980;65(1):404–411. doi: 10.1016/s0076-6879(80)65051-4. [DOI] [PubMed] [Google Scholar]
  6. Edwards Y. H., Parkar M., Povey S., West L. F., Parrington J. M., Solomon E. Human myosin heavy chain genes assigned to chromosome 17 using a human cDNA clone as probe. Ann Hum Genet. 1985 May;49(Pt 2):101–109. doi: 10.1111/j.1469-1809.1985.tb01681.x. [DOI] [PubMed] [Google Scholar]
  7. Efstratiadis A., Posakony J. W., Maniatis T., Lawn R. M., O'Connell C., Spritz R. A., DeRiel J. K., Forget B. G., Weissman S. M., Slightom J. L. The structure and evolution of the human beta-globin gene family. Cell. 1980 Oct;21(3):653–668. doi: 10.1016/0092-8674(80)90429-8. [DOI] [PubMed] [Google Scholar]
  8. Feghali R., Leinwand L. A. Molecular genetic characterization of a developmentally regulated human perinatal myosin heavy chain. J Cell Biol. 1989 May;108(5):1791–1797. doi: 10.1083/jcb.108.5.1791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  10. Geisterfer-Lowrance A. A., Kass S., Tanigawa G., Vosberg H. P., McKenna W., Seidman C. E., Seidman J. G. A molecular basis for familial hypertrophic cardiomyopathy: a beta cardiac myosin heavy chain gene missense mutation. Cell. 1990 Sep 7;62(5):999–1006. doi: 10.1016/0092-8674(90)90274-i. [DOI] [PubMed] [Google Scholar]
  11. Green E. D., Olson M. V. Chromosomal region of the cystic fibrosis gene in yeast artificial chromosomes: a model for human genome mapping. Science. 1990 Oct 5;250(4977):94–98. doi: 10.1126/science.2218515. [DOI] [PubMed] [Google Scholar]
  12. Green E. D., Olson M. V. Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1213–1217. doi: 10.1073/pnas.87.3.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gulick J., Subramaniam A., Neumann J., Robbins J. Isolation and characterization of the mouse cardiac myosin heavy chain genes. J Biol Chem. 1991 May 15;266(14):9180–9185. [PubMed] [Google Scholar]
  14. Hart C. P., Fainsod A., Ruddle F. H. Sequence analysis of the murine Hox-2.2, -2.3, and -2.4 homeo boxes: evolutionary and structural comparisons. Genomics. 1987 Oct;1(2):182–195. doi: 10.1016/0888-7543(87)90011-5. [DOI] [PubMed] [Google Scholar]
  15. Karsch-Mizrachi I., Feghali R., Shows T. B., Leinwand L. A. Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA. Gene. 1990 May 14;89(2):289–294. doi: 10.1016/0378-1119(90)90020-r. [DOI] [PubMed] [Google Scholar]
  16. Karsch-Mizrachi I., Travis M., Blau H., Leinwand L. A. Expression and DNA sequence analysis of a human embryonic skeletal muscle myosin heavy chain gene. Nucleic Acids Res. 1989 Aug 11;17(15):6167–6179. doi: 10.1093/nar/17.15.6167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kraft R., Tardiff J., Krauter K. S., Leinwand L. A. Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase. Biotechniques. 1988 Jun;6(6):544-6, 549. [PubMed] [Google Scholar]
  18. Ledbetter S. A., Nelson D. L., Warren S. T., Ledbetter D. H. Rapid isolation of DNA probes within specific chromosome regions by interspersed repetitive sequence polymerase chain reaction. Genomics. 1990 Mar;6(3):475–481. doi: 10.1016/0888-7543(90)90477-c. [DOI] [PubMed] [Google Scholar]
  19. Leinwand L. A., Fournier R. E., Nadal-Ginard B., Shows T. B. Multigene family for sarcomeric myosin heavy chain in mouse and human DNA: localization on a single chromosome. Science. 1983 Aug 19;221(4612):766–769. doi: 10.1126/science.6879174. [DOI] [PubMed] [Google Scholar]
  20. Leinwand L. A., Saez L., McNally E., Nadal-Ginard B. Isolation and characterization of human myosin heavy chain genes. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3716–3720. doi: 10.1073/pnas.80.12.3716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lyons G. E., Ontell M., Cox R., Sassoon D., Buckingham M. The expression of myosin genes in developing skeletal muscle in the mouse embryo. J Cell Biol. 1990 Oct;111(4):1465–1476. doi: 10.1083/jcb.111.4.1465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Parker-Thornburg J., Bauer B., Palermo J., Robbins J. Structural and developmental analysis of two linked myosin heavy chain genes. Dev Biol. 1992 Mar;150(1):99–107. doi: 10.1016/0012-1606(92)90010-e. [DOI] [PubMed] [Google Scholar]
  23. Pette D., Staron R. S. Cellular and molecular diversities of mammalian skeletal muscle fibers. Rev Physiol Biochem Pharmacol. 1990;116:1–76. doi: 10.1007/3540528806_3. [DOI] [PubMed] [Google Scholar]
  24. Riley J., Butler R., Ogilvie D., Finniear R., Jenner D., Powell S., Anand R., Smith J. C., Markham A. F. A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res. 1990 May 25;18(10):2887–2890. doi: 10.1093/nar/18.10.2887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Robert B., Barton P., Minty A., Daubas P., Weydert A., Bonhomme F., Catalan J., Chazottes D., Guénet J. L., Buckingham M. Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross. Nature. 1985 Mar 14;314(6007):181–183. doi: 10.1038/314181a0. [DOI] [PubMed] [Google Scholar]
  26. Rubinstein N. A., Kelly A. M. Development of muscle fiber specialization in the rat hindlimb. J Cell Biol. 1981 Jul;90(1):128–144. doi: 10.1083/jcb.90.1.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Saez L. J., Gianola K. M., McNally E. M., Feghali R., Eddy R., Shows T. B., Leinwand L. A. Human cardiac myosin heavy chain genes and their linkage in the genome. Nucleic Acids Res. 1987 Jul 10;15(13):5443–5459. doi: 10.1093/nar/15.13.5443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Saez L., Leinwand L. A. Characterization of diverse forms of myosin heavy chain expressed in adult human skeletal muscle. Nucleic Acids Res. 1986 Apr 11;14(7):2951–2969. doi: 10.1093/nar/14.7.2951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schwartz C. E., McNally E., Leinwand L., Skolnick M. H. A polymorphic human myosin heavy chain locus is linked to an anonymous single copy locus (D17S1) at 17p13. Cytogenet Cell Genet. 1986;43(1-2):117–120. doi: 10.1159/000132307. [DOI] [PubMed] [Google Scholar]
  30. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  31. Silverman G. A., Ye R. D., Pollock K. M., Sadler J. E., Korsmeyer S. J. Use of yeast artificial chromosome clones for mapping and walking within human chromosome segment 18q21.3. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7485–7489. doi: 10.1073/pnas.86.19.7485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Subramaniam A., Jones W. K., Gulick J., Wert S., Neumann J., Robbins J. Tissue-specific regulation of the alpha-myosin heavy chain gene promoter in transgenic mice. J Biol Chem. 1991 Dec 25;266(36):24613–24620. [PubMed] [Google Scholar]
  33. Tsika R. W., Bahl J. J., Leinwand L. A., Morkin E. Thyroid hormone regulates expression of a transfected human alpha-myosin heavy-chain fusion gene in fetal rat heart cells. Proc Natl Acad Sci U S A. 1990 Jan;87(1):379–383. doi: 10.1073/pnas.87.1.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Weydert A., Daubas P., Caravatti M., Minty A., Bugaisky G., Cohen A., Robert B., Buckingham M. Sequential accumulation of mRNAs encoding different myosin heavy chain isoforms during skeletal muscle development in vivo detected with a recombinant plasmid identified as coding for an adult fast myosin heavy chain from mouse skeletal muscle. J Biol Chem. 1983 Nov 25;258(22):13867–13874. [PubMed] [Google Scholar]
  35. Weydert A., Daubas P., Lazaridis I., Barton P., Garner I., Leader D. P., Bonhomme F., Catalan J., Simon D., Guénet J. L. Genes for skeletal muscle myosin heavy chains are clustered and are not located on the same mouse chromosome as a cardiac myosin heavy chain gene. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7183–7187. doi: 10.1073/pnas.82.21.7183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Whalen R. G., Sell S. M., Butler-Browne G. S., Schwartz K., Bouveret P., Pinset-Härstöm I. Three myosin heavy-chain isozymes appear sequentially in rat muscle development. Nature. 1981 Aug 27;292(5826):805–809. doi: 10.1038/292805a0. [DOI] [PubMed] [Google Scholar]
  37. Wydro R. M., Nguyen H. T., Gubits R. M., Nadal-Ginard B. Characterization of sarcomeric myosin heavy chain genes. J Biol Chem. 1983 Jan 10;258(1):670–678. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES