NREM sleep increases across the light-phase two days after remote preconditioning. (A) Representative hypnograms of wake (W), NREM sleep (N), and REM sleep (R) across the 12 h light-phase/12 h dark-phase in preconditioned mice (remote preconditioning) and sham (anesthetized) mice. ZT 12, lights-off; ZT 24, lights-on. Means ± SEM percent time spent awake or asleep across the 12 h dark-phase (B) and 12 h light-phase (D) and time spent in either REM or NREM sleep across the 12 h dark-phase (C) and 12 h light-phase (E). **P < 0.05, two-way ANOVA with Dunn-Sidak multiple comparison. n = 5 per treatment.