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. 2016 Oct 18;18(11):654–665. doi: 10.1016/j.neo.2016.09.004

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Supplementary Figure S2 — Effects of KRAS knockdown in CRC cell lines. (A) Quantitative RT-PCR showing relative mRNA levels for KRAS. HCT116 cells (left) and DLD-1 cells (right) were treated separately with two independent siRNA constructs (#1 and #2) targeting KRAS and NC siRNA. Mean; bars, ± SD, n = 3 (Student's t test; *P < .05). (B) Western blot photographs of KRAS, ASNS, and β-actin (Actin). HCT116 cells were treated separately with two independent siRNA constructs (#1 and #2) targeting KRAS and NC siRNA.