Fig. 9.

Hydrolysis of 5-FT UDP-Gal (1) into 5-FT UMP (3) upon incubation with hPBMCs. Cells were incubated with 1 (1 mM) over 24 h and samples taken at different time points. The relative amounts of intact 1 and its metabolite 3 are shown as percentages of the total amount (1 + 3) in, respectively, the supernatant (extracellular fraction, from supernatant), or the cell pellet after centrifugation and lysis (intracellular fraction, from cell pellet). Values are mean ± SEM from 4 independent experiments using hPBMCs from 2 human donors.