Figure 2.

Time course of reaction of Op maltase with (A) 50 mm 1‐kestose, (B) 50 mm melezitose, (C) 2% malt extract and (D) 2% isomalto‐oligosaccharides, analysed by TLC. The reaction was conducted in maltase buffer, using 13 µg/ml maltase protein (1 U/ml): from top to bottom, M1, fructose, sucrose, 1‐kestose, nystose; M2, glucose, maltose, maltotriose, stachyose; M3, turanose; M4, glucose, maltose, maltotriose; M5, isomaltose, panose; M6, malt extract. References (R) designate (A) 50 mm 1‐kestose; (B) 50 mm melezitose; (C) 2% malt extract; and (D) 2% isomalto‐oligosaccharides (IMOs), respectively, without the enzyme added; G, glucose; IB, inulobiose; 1‐K, 1‐kestose; F, fructose; S, sucrose; MZ, melezitose; M, maltose; MT, maltotriose; IM, isomaltose; P, panose