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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Biomaterials. 2016 Sep 30;111:66–79. doi: 10.1016/j.biomaterials.2016.09.024

Figure 5. Effects of switch between static and dynamic culture on structure and function of NRVM cardiobundles.

Figure 5

(A–B) Max twitch force (A) and CV (B) at 2 Hz pacing in static (Stat) and dynamic (Dyn) cardiobundles on culture day 7 and 14, cardiobundles cultured static for 7 days then dynamic for 7 days (Stat→Dyn), and cardiobundles cultured dynamic for 7 days then static for 7 days (Dyn→Stat) (n = 12–14 cardiobundles per group from 3 cell isolations). (C) Representative cross-sections of cardiobundles stained for F-actin (green) and nuclei (DAPI, blue). (D–F) Quantification of D) total and F-actin+ area, E) total and F-actin+ nuclei, and F) F-actin+ area per F-actin+ nucleus in cardiobundle cross-sections (n = 5–7 cardiobundles per group from 2 cell isolations). *, p < 0.05 vs. static at same culture point; #, p < 0.05 vs. dynamic at the same culture point.