Figure 2.

Validation of the siRNA library screening. A, Seventeen genes were randomly selected from the 80 identified in the screen, and expression was knocked down in primary cultured cortical neurons (7 days in vitro) by lentiviral siRNAs for the 17 genes. B, Representative images of neurons treated with lethal OGD (90 min) and stained with Hoechst 33342 and propidium iodide (PI). Dead neurons were scored as red cells with condensed or fragmented nuclei. Non-virus-infected neurons were used as viral control, and neurons infected with lentiviral siRNA for DsRed were used as siRNA control. Scale bar =100 µm. C, Quantification of cell death in B. Experiments were performed three times, and data represent the mean ± SEM. Statistical significance from DsRed siRNA control indicated at *p < 0.05; differences between multiple groups were evaluated by one-way ANOVA followed by the Tukey–Kramer post hoc test.