Figure 2. Rapamycin inhibits NF-κB activation HNSCC.

A. Cells were treated with increasing doses of Rapamycin for 1 hour and then cells were lysed. The levels of p65, Akt, S6K and of endogenous phosphorylation of S6K, p65, and Akt were determined by probing with the indicated antibodies. The experiments were repeated for three times. B. Cells were treated with 300 nM Rapamycin for 1-4 hours and then cells were lysed for western bot.