Figure 5. EGFR induction of NF-κB is mTORC1 dependent in HNSCC.

A. Overexpression of EGFR increases activity of Akt, mTORC1, and NF-κB. EGFR was transfected in UMSCC25, Cal27 and UMSCC1 cells and the phosphorylation of Akt, S6K and p65, and total Akt, S6K and p65, as well as EGFR expression were tested by western blot. B. Depletion of Raptor blocks EGFR induction of NF-κB. Cells were transfected with non-target siRNA or siRNA against Raptor for 48 hours and then EGFR were transfected for another 24 hours. Cells were lysed and the levels of p65, Akt, S6K and of endogenous phosphorylation of S6K, p65, Akt2 and Raptor, as well as total EGFR were determined by probing with the indicated antibodies. All experiments were repeated for three times.