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. 2016 Apr 1;7(22):32088–32099. doi: 10.18632/oncotarget.8525

Figure 1. TR4 was up-regulated in tumor tissues among HCC patients.

Figure 1

(A) TR4 mRNA levels in 14 HCC patients. TR4 mRNA levels in tumor and surrounding non-tumor tissues were evaluated using real-time RT-PCR analysis as indicated, GAPDH showed stable expression in the conditions applied and was used as a reference gene. Mean threshold cycle (Ct) number of triplicate runs were used for data analysis. The relative expression of TR4 was calculated compared with the reference gene (GAPDH). (B) TR4 protein expression levels in tumor (T) and surrounding non-tumor (N) tissues were evaluated using Western blot analysis in another 12 patients as indicated. GAPDH served as a loading control. (C) TR4 staining in non-tumor lesions (upper panel), with less in tumor lesions (lower panel). All assays were performed in triplicate (***P < 0.001).