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. 2016 Apr 18;7(22):32956–32968. doi: 10.18632/oncotarget.8793

Figure 4. Overlap of genes regulated by MCPyV-TA and RB1 in MCC cells.

Figure 4

TA-shRNA was expressed in WaGa cells stably transduced with either empty vector, vector coding for MCPyV-TA, a Scr-shRNA vector or an RB1-shRNA construct. After 5 days LT and RB1 protein levels were analyzed by immunoblot, and mRNA expression levels of 245 cancer related genes were analyzed using the NanoString nCounter™ gene expression system [24]. 90 genes were excluded from further analysis due to very low expression. The absolute expression values of the remaining 155 genes were normalized to the mean value of the 6 house keeping genes. Depicted are the relative mRNA expression levels, i.e. TA-shRNA expressing cells relative to their controls, of the 21 genes displaying a more than two fold change in the empty vector and the Scr-shRNA cells.