Skip to main content
. 2016 May 7;7(22):33363–33373. doi: 10.18632/oncotarget.9221

Figure 1. KSHV miR-K12-1 induces NF-κB and STAT3 activation and cell transformation.

Figure 1

A. miR-K12-1 represses RNA and protein expression of IκBα. Real-time RT-PCR and immunoblotting analyses were performed to examine the IκBα RNA and protein levels, respectively, in Hela cells transfected with miR-K12-1 or an empty vector. B. miR-K12-1 induces RelA nuclear translocation. Immunofluorescence assay was performed to examine the nuclear translocation of RelA protein in Hela cells transfected with miR-K12-1 or an empty vector. Scale bar: 10 μm. C. miR-K12-1 induces transcriptional activation of NF-κB. Luciferase assay was performed to measure NF-κB transcriptional activity in Hela cells transfected with miR-K12-1 or an empty vector, together with NF-κB luciferase reporter. D. miR-K12-1 induces RelA and STAT3 nuclear expression. Nuclear fraction immunoblotting was performed to examine the nuclear expression of RelA and STAT3 proteins in Hela cells transfected with miR-K12-1 or an empty vector. E. miR-K12-1 induces STAT3 nuclear translocation. Immunofluorescence assay was performed to examine the nuclear translocation of STAT3 protein in Hela cells transfected with miR-K12-1 or an empty vector. Scale bar: 10 μm. F. miR-K12-1 induces transcriptional activation of STAT3. Luciferase assay was performed to measure STAT3 transcriptional activity in Hela cells transfected with miR-K12-1 or an empty vector, together with STAT3 luciferase reporter. G. miR-K12-1 promotes cell growth. Cell growth rates were determined for Rat-1 cells stably expressing miR-K12-1 or an empty vector. H. miR-K12-1 induces cell transformation. Soft agar colony formation assay was performed for Rat-1 cells stably expressing miR-K12-1 or an empty vector.