Skip to main content
. 2016 May 7;7(22):33363–33373. doi: 10.18632/oncotarget.9221

Figure 4. KSHV oncogenicity involves activation of miR-K12-1/NF-κB/IL-6/STAT3 signaling.

Figure 4

A. RelA knockdown decreases STAT3 nuclear expression in BCBL-1 cells. Nuclear fraction immunoblotting assay was performed to examine the nuclear expression of RelA and STAT3 proteins in BCBL-1 cells stably expressing RelA shRNA or an empty vector. B. RelA knockdown suppresses IL-6 but not STAT3 expression in BCBL-1 cells. Real time PCR analysis was performed to detect RelA, IL-6 and STAT3 RNA expression levels in BCBL-1 cells stably expressing RelA shRNA or an empty vector. C. IL-6 knockdown blocks STAT3 nuclear expression in BCBL-1 cells. Nuclear fraction immunoblotting assay was performed to examine the nuclear expression of RelA and STAT3 proteins in BCBL-1 cells stably expressing IL-6 shRNA or an empty vector. D. KSHV requires miR-K12-1 to induce NF-κB and STAT3 activation. Luciferase reporter activity assay was performed to examine the activation of NF-κB and STAT3 in 293FT cells with an empty vector, KSHVBac36, KSHVBac36 ΔmiRNA, or KSHVBac36 ΔmiRNA plus miR-K12-1. E. Knockdown of RelA, IL-6 or STAT3 suppresses the oncogenicty of BCBL-1 cells. Soft agar colony formation assay was performed for BCBL-1 cells stably expressing RelA shRNA, IL-6 shRNA, STAT3 shRNA, or an empty vector. F. RelA knockdown suppresses expression of cell survival and proliferation genes in BCBL-1 cells. Real time PCR analysis was performed to detect RNA expression levels of the indicated genes in BCBL-1 cells stably expressing RelA shRNA or an empty vector. G. STAT3 knockdown suppresses expression of cell survival and proliferation genes in BCBL-1 cells. Real time PCR analysis was performed to detect RNA expression levels of the indicated genes in BCBL-1 cells stably expressing STAT3 shRNA or an empty vector.