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. 1997 Feb 15;99(4):592–595. doi: 10.1172/JCI119200

Total energy expenditure and the level of physical activity correlate with plasma leptin concentrations in five-year-old children.

A D Salbe 1, M Nicolson 1, E Ravussin 1
PMCID: PMC507839  PMID: 9045859

Abstract

Leptin, the product of the ob gene, is a hormone secreted by adipocytes that is known to decrease food intake and increase energy expenditure in ob/ob mice. In humans, variants in the OB gene have not been detected and very little is known about the action of leptin on food intake and energy expenditure, although circulating leptin concentrations are positively correlated to body fat stores. The purpose of this study was to assess the relationship between fasting plasma leptin concentrations and energy expenditure in 123 5-yr-old Pima Indian children (67 males/76 females). Body composition was assessed by isotopic water dilution (18O) whereas total energy expenditure (TEE) and resting metabolic rate (RMR) were measured using doubly labeled water and indirect calorimetry, respectively. The physical activity level was calculated as the ratio of TEE:RMR. Plasma leptin concentrations were positively correlated to percent body fat (r = 0.84, P < 0.0001), but were similar in boys and girls after adjusting for percent body fat. Most importantly, we found that, independent of the percentage of body fat, plasma leptin concentrations correlated with TEE (in absolute values, r = 0.37, P < 0.0001, or adjusted for body size r = 0.42; P < 0.0001) and with physical activity level (r = 0.26, P < 0.01), but not RMR. These results suggest that, as in animal models, leptin plays a role in energy expenditure in humans.

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Selected References

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  1. Campfield L. A., Smith F. J., Guisez Y., Devos R., Burn P. Recombinant mouse OB protein: evidence for a peripheral signal linking adiposity and central neural networks. Science. 1995 Jul 28;269(5223):546–549. doi: 10.1126/science.7624778. [DOI] [PubMed] [Google Scholar]
  2. Christin L., O'Connell M., Bogardus C., Danforth E., Jr, Ravussin E. Norepinephrine turnover and energy expenditure in Pima Indian and white men. Metabolism. 1993 Jun;42(6):723–729. doi: 10.1016/0026-0495(93)90239-k. [DOI] [PubMed] [Google Scholar]
  3. Collins S., Kuhn C. M., Petro A. E., Swick A. G., Chrunyk B. A., Surwit R. S. Role of leptin in fat regulation. Nature. 1996 Apr 25;380(6576):677–677. doi: 10.1038/380677a0. [DOI] [PubMed] [Google Scholar]
  4. Considine R. V., Sinha M. K., Heiman M. L., Kriauciunas A., Stephens T. W., Nyce M. R., Ohannesian J. P., Marco C. C., McKee L. J., Bauer T. L. Serum immunoreactive-leptin concentrations in normal-weight and obese humans. N Engl J Med. 1996 Feb 1;334(5):292–295. doi: 10.1056/NEJM199602013340503. [DOI] [PubMed] [Google Scholar]
  5. Fomon S. J., Haschke F., Ziegler E. E., Nelson S. E. Body composition of reference children from birth to age 10 years. Am J Clin Nutr. 1982 May;35(5 Suppl):1169–1175. doi: 10.1093/ajcn/35.5.1169. [DOI] [PubMed] [Google Scholar]
  6. Fontvieille A. M., Dwyer J., Ravussin E. Resting metabolic rate and body composition of Pima Indian and Caucasian children. Int J Obes Relat Metab Disord. 1992 Aug;16(8):535–542. [PubMed] [Google Scholar]
  7. Fontvieille A. M., Harper I. T., Ferraro R. T., Spraul M., Ravussin E. Daily energy expenditure by five-year-old children, measured by doubly labeled water. J Pediatr. 1993 Aug;123(2):200–207. doi: 10.1016/s0022-3476(05)81689-0. [DOI] [PubMed] [Google Scholar]
  8. Halaas J. L., Gajiwala K. S., Maffei M., Cohen S. L., Chait B. T., Rabinowitz D., Lallone R. L., Burley S. K., Friedman J. M. Weight-reducing effects of the plasma protein encoded by the obese gene. Science. 1995 Jul 28;269(5223):543–546. doi: 10.1126/science.7624777. [DOI] [PubMed] [Google Scholar]
  9. Maffei M., Halaas J., Ravussin E., Pratley R. E., Lee G. H., Zhang Y., Fei H., Kim S., Lallone R., Ranganathan S. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat Med. 1995 Nov;1(11):1155–1161. doi: 10.1038/nm1195-1155. [DOI] [PubMed] [Google Scholar]
  10. Maffei M., Stoffel M., Barone M., Moon B., Dammerman M., Ravussin E., Bogardus C., Ludwig D. S., Flier J. S., Talley M. Absence of mutations in the human OB gene in obese/diabetic subjects. Diabetes. 1996 May;45(5):679–682. doi: 10.2337/diab.45.5.679. [DOI] [PubMed] [Google Scholar]
  11. Mantzoros C. S., Qu D., Frederich R. C., Susulic V. S., Lowell B. B., Maratos-Flier E., Flier J. S. Activation of beta(3) adrenergic receptors suppresses leptin expression and mediates a leptin-independent inhibition of food intake in mice. Diabetes. 1996 Jul;45(7):909–914. doi: 10.2337/diab.45.7.909. [DOI] [PubMed] [Google Scholar]
  12. Pelleymounter M. A., Cullen M. J., Baker M. B., Hecht R., Winters D., Boone T., Collins F. Effects of the obese gene product on body weight regulation in ob/ob mice. Science. 1995 Jul 28;269(5223):540–543. doi: 10.1126/science.7624776. [DOI] [PubMed] [Google Scholar]
  13. Ravussin E., Harper I. T., Rising R., Bogardus C. Energy expenditure by doubly labeled water: validation in lean and obese subjects. Am J Physiol. 1991 Sep;261(3 Pt 1):E402–E409. doi: 10.1152/ajpendo.1991.261.3.E402. [DOI] [PubMed] [Google Scholar]
  14. Ravussin E., Lillioja S., Anderson T. E., Christin L., Bogardus C. Determinants of 24-hour energy expenditure in man. Methods and results using a respiratory chamber. J Clin Invest. 1986 Dec;78(6):1568–1578. doi: 10.1172/JCI112749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rohner-Jeanrenaud F., Jeanrenaud B. Obesity, leptin, and the brain. N Engl J Med. 1996 Feb 1;334(5):324–325. doi: 10.1056/NEJM199602013340511. [DOI] [PubMed] [Google Scholar]
  16. Schwartz M. W., Baskin D. G., Bukowski T. R., Kuijper J. L., Foster D., Lasser G., Prunkard D. E., Porte D., Jr, Woods S. C., Seeley R. J. Specificity of leptin action on elevated blood glucose levels and hypothalamic neuropeptide Y gene expression in ob/ob mice. Diabetes. 1996 Apr;45(4):531–535. doi: 10.2337/diab.45.4.531. [DOI] [PubMed] [Google Scholar]
  17. Stephens T. W., Basinski M., Bristow P. K., Bue-Valleskey J. M., Burgett S. G., Craft L., Hale J., Hoffmann J., Hsiung H. M., Kriauciunas A. The role of neuropeptide Y in the antiobesity action of the obese gene product. Nature. 1995 Oct 12;377(6549):530–532. doi: 10.1038/377530a0. [DOI] [PubMed] [Google Scholar]
  18. Wallin B. G., Kunimoto M. M., Sellgren J. Possible genetic influence on the strength of human muscle nerve sympathetic activity at rest. Hypertension. 1993 Sep;22(3):282–284. doi: 10.1161/01.hyp.22.3.282. [DOI] [PubMed] [Google Scholar]
  19. Zhang Y., Proenca R., Maffei M., Barone M., Leopold L., Friedman J. M. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994 Dec 1;372(6505):425–432. doi: 10.1038/372425a0. [DOI] [PubMed] [Google Scholar]
  20. Zurlo F., Ferraro R. T., Fontvielle A. M., Rising R., Bogardus C., Ravussin E. Spontaneous physical activity and obesity: cross-sectional and longitudinal studies in Pima Indians. Am J Physiol. 1992 Aug;263(2 Pt 1):E296–E300. doi: 10.1152/ajpendo.1992.263.2.E296. [DOI] [PubMed] [Google Scholar]

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