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The dual luciferase reporter assay of miR-137 mimics/negative co-transfecting with PmirGLO + ZNF804A in HEK293T cells. 24 h after transfection, the cells were assayed for firefly luciferase activity and Renilla luciferase activity test and the ratio of firefly/Renilla is obtained. Bars represent SEM from three experiments.
NC, negative control. ** indicates P < 0.01.
