Figure 5.

Overexpression of PRKAR1β reverses the effect of hyperglycemic culture. ECFCs grown in 25 mmol/L d-glucose for 5 days were transfected with PRKAR1β (R1β) or control vector (V) and lysed 2 days later. A: PKA activity. The PKA activity of the d-glucose–cultured transfectants was determined as outlined in research design and methods. n = 4. *P < 0.05. B: α4 Phosphorylation (p-α4). Levels of phosphorylated α4-integrin in the transfectants were determined by Western blotting. Illustrated is a representative blot. C: Adhesion. The adhesive properties of the d-glucose–cultured transfectants to an S17 cell monolayer were determined and grouped data illustrated. n = 6. *P < 0.05.