Figure 1.

Loss of NNT increases JH₂O₂ emitting potential in permeabilized fibers. Permeabilized fibers were prepared from RG of 6N and 6J mice. JH₂O₂ emission was assessed via the Amplex Ultra Red/horseradish peroxidase system, and resorufin fluorescence was converted to pmoles of H₂O₂ via an H₂O₂ standard curve. A: Representative trace of an JH₂O₂ emission experiment performed in the presence of pyruvate (Pyr; 1 mmol) plus carnitine (Carn; 5 mmol) in 6N and 6J fibers. After 5 min, carmustine (BCNU; 100 μmol) and AF (1 μmol) were added to inhibit peroxide buffering. Numbers above each trace section correspond to the mean rates of JH₂O₂ emission (pmol/s/mg dry weight) for that condition from N = 5 experimental replicates. B and C: Same experimental design as in A, except substrate condition was succinate (10 mmol) (B) or pyruvate (1 mmol) plus malate (2 mmol) (C). For C, the first number listed is for 6N mice, followed by 6J.