Figure 6.

Increased hepatic FAO and CL synthesis in TAZ kd mice. A–F: Primary cultures of hepatocytes were isolated from 4-month-old NTg and Tg mice fed a diet containing dox. Cells were incubated with [¹⁴C]-oleate for the durations indicated, and radiolabel was measured in the media as acid-soluble metabolites (ASMs; n = 5–6) (A) and in total cell lysate (n = 5–6) (B). Hepatocytes were incubated with [¹⁴C]-oleate (C and E) or [¹⁴C] acetate (D and F) for the durations indicated, and the incorporation of radiolabel into TG and CL was measured (n = 4–6). G: Immunoblot of MTPa, TAM41, ALCAT1, cardiolipin synthase (CLS), uncoupling protein 2 (UCP2), CD36, hormone-sensitive lipase (HSL), and loading control (protein disulfide isomerase [PDI]) in liver lysates and CDP-diacylglycerol synthase 2 (CDS2) and a loading control (citrate synthase) in isolated liver mitochondria (20 μg protein). H and I: Quantitation of immunoblots by densitometry with normalization against PDI (n = 3–6). J: The mRNA levels of the indicated genes measured in the liver by quantitative PCR. DPM, disintegrations per minute; rel., relative. All data are means ± SEMs (n = 3–7). *P < 0.05 compared with NTg age-matched mice fed an identical diet; †P < 0.05, Tg +dox compared to all other groups; ‡P < 0.05, NTg +dox compared to all other groups.