Fig. 4.

Immunofluorescence study of the effects of paliperidone on microglia M2 cellular markers after acute restraint stress exposure. Arginase (ArgI) immunoreactivity in 30-μm-thick sections through the brain prefrontal cortex of rats in (A–F) control and (G–L) acute restraint stress conditions, and pretreated with (A–C, G–I) vehicle or (D–F, J–L) paliperidone. Microglia cellular marker ionized calcium binding adaptor molecule 1 (appears in green and ArgI in red in all cases. In the particular cases, ArgI localizes to the respective cellular marker with areas of overlap appearing yellow/orange in the merged image (white arrows) Scale bar = 50 μm