Figure 4. YAP cellular accumulation in shGLO1 MDA-MB-231 clones.
(A) GLO1 mRNA, (B) protein and (C) activity level in MDA-MB-231 shNT control and shGLO1#1 and #2. (D) YAP immunofluorescence (Santa Cruz antibody, H125) in MDA-MB-231 silenced for GLO1 (shGLO1#1 and #2) cultured from low to high density. Detection of MG was performed using MBo-specific fluorescent probe. Data are representative of three independent experiments. Magnification 630x. Zoomed pictures are shown when indicated. (E) Quantification of nuclear YAP corresponding to D experiment. All data were analyzed using one-way ANOVA followed by Dunnett post-test and shown as the mean values ± SEM of at least three independent experiments. *p<0.05, **p<0.01 and ***p<0.001.
Figure 4—figure supplement 1. Carnosine and aminoguanidine MG scavengers reverse YAP accumulation in GLO1-depleted MDA-MB-231.
(A) TAZ immunofluorescence staining in GLO1 depleted MDA-MB-231 cells. Magnification 630x. Data are representative of two independent experiments. (B) MDA-MB-231 silenced for GLO1 (shGLO1#1 and #2) cells cultured until they reached high density and treated with carnosine (10 mM) or aminoguanidine (10 mM) impeded cellular accumulation of YAP. Magnification 630x. Data are representative of two independent experiments.