MICROBIOLOGY Correction for “Production of functional small interfering RNAs by an amino-terminal deletion mutant of human Dicer,” by Edward M. Kennedy, Adam W. Whisnant, Anand V. R. Kornepati, Joy B. Marshall, Hal P. Bogerd, and Bryan R. Cullen, which appeared in issue 50, December 15, 2015, of Proc Natl Acad Sci USA (112:E6945–E6954; first published November 30, 2015; 10.1073/pnas.1513421112).
The authors note that there are errors in the published Fig. 5. Specifically, the influenza A virus (IAV) small RNA-seq data presented in panels A, B, and C are given with the polarity of the reads reversed. Second, the data portrayed in Fig. 5D was erroneously graphed using partially incorrect small RNA deep sequencing data sets derived using a mutant form of IAV not discussed in this article. The figure has been corrected using the correct, matched data sets only. Finally, during our effort to correct these errors, we recently observed that the IAV A/Puerto Rico/8/34/Mount Sinai strain used in our experiments contains a small number of sequence polymorphisms relative to the viral genome sequence (EF1909790-EF190986) used for read alignment in our initial analysis. We have now repeated the analysis of the obtained small RNA sequence data aligning to the correct viral genome sequence (AF389115.1-AF389122.1). Moreover, we have now sequenced the IAV strain we used to identify any additional polymorphisms that might have arisen during virus propagation. The corrected Fig. 5 and its legend appear below.
Fig. 5.
Detection of viral siRNAs in IAV infected human cells. NoDice/ΔPKR cells were transfected and blasticidin-selected, as described in Fig. 4, and were then infected with IAV for 24 h at an MOI of 2.0. (A–C) Length distribution of small RNA reads aligning to the IAV genome, normalized to total read numbers given in counts per million, for cells lacking hDcr or expressing ectopic WT or N1 hDcr. (D) Coverage plots for IAV small RNA reads (22 ± 2 nt) aligning to IAV genome segments PB2, PB1, or PA and given in counts per million. (E) NoDice/ ΔPKR cells expressing either the WT or N1 forms of hDcr were infected with IAV at the indicated MOI and total RNA harvested at 24-h postinfection. This bar graph depicts the expression level of IAV transcripts encoding the viral NP gene as determined by qRT-PCR. RNA levels were normalized to endogenous human β-actin mRNA and are given relative to the IAV NP RNA level detected in control vector-transfected NoDice/ΔPKR cells, which was set at 1.0. Average of three independent experiments with SD and significance indicated.
Please note that these errors do not affect the conclusions drawn from Fig. 5, or from the article in general. We sincerely apologize for these errors, and for any resulting confusion.