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. 2016 Jun 16;12(9):1575–1592. doi: 10.1080/15548627.2016.1192750

Figure 1.

Figure 1.

LC3A/B and ATG7 are mainly expressed and localized in the elongating spermatids. (A) Immunofluorescent staining (IF) of LC3A/B protein in testes of mice at postnatal d 7, d 15, d 20, and d 36. Blue, DAPI; green, LC3A/B. The enlarged images originated from the dotted squares highlighting green LC3A/B expression and localization in the elongating spermatids. The right panel model shows the schematic of LC3A/B expression in the testes of the indicated age, green markers LC3A/B localization. (B) Immunohistochemistry (IHC) of LC3A/B in 8-wk-old mouse testes. (C) The IHC of ATG7 in 8-wk-old mouse testes. The framed area was magnified. Sertoli, Sertoli cells; Spg-A, spermatogonium A; Spg-B, spermatogonium B; Spc, spermatocyte; RS, round spermatid; ES, elongating spermatid.