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. 2016 Jun 16;12(9):1575–1592. doi: 10.1080/15548627.2016.1192750

Figure 5.

Figure 5.

Knockdown of Pdlim1 in atg7-IKO MEFs partially rescues the cytoskeleton organization defect. (A) Infection of Pdlim1 shRNA1 virus inhibited the expression of PDLIM1 protein in both Atg7+/+ and atg7-IKO MEFs. The uninfected cells in the same dish served as an internal control. (B) The knockdown efficiency of Pdlim1 by shRNA, detected by immunoblotting. (C) Knockdown of Pdlim1 by shRNA1 in atg7-IKO MEFs partially rescued the disordered F-actin networks (lower panel, red dotted area), but has no influence on Atg7+/+ MEFs (upper panel red dotted). The uninfected cells in the same dish served as an internal control (white dotted areas). (D) Quantification of the rescue efficiency of Pdlim1 knockdown.