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. 2016 Apr 23;7(23):34571–34581. doi: 10.18632/oncotarget.8952

Figure 5. Effects of HIF-2α shRNA on the promoter activities of PDCD4, Bak, ZBP-89 in HepG2 and Hep3B cells.

Figure 5

HepG2 and Hep3B cells were transiently transfected with 0.4 μg of individual (ZBP-89 or Bak or PDCD4) promoter luciferase construct, pGL3 Basic or pGL3 control plasmids, together with 10 ng Renilla luciferase plasmid which served as an inner control for transfection efficiency and 0.8 μg pshRNA-control or pshRNA-HIF-2α. The cells were lysed to determinate the relative firefly luciferase activity and the change of the activity was calculated in fold. The results were the mean of 3 independent experiments, and the values are expressed as mean ±SD (**P<0.01).