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. 2016 Apr 25;7(23):34617–34629. doi: 10.18632/oncotarget.8964

Figure 3. FXR agonist GW4064/CDDP co-treatment additively inhibits Bcl-xl expression.

Figure 3

A. Protein levels of Bax, Bak, Bcl-2, MCL1 and Bcl-xL in GBC-SD cells treated with CDDP alone, GW4064 alone and CDDP/GW4064 combination for 36h. B. Protein levels of Bcl-2 and Bcl-xL in RBE cells treated with CDDP alone, GW4064 alone and CDDP/GW4064 combination for 36h. C, D. The mRNA levels of Bcl-xL in GBC-SD (C) and RBE (D) cells treated with CDDP alone, GW4064 alone and CDDP/GW4064 combination for 24h. Columns, mean of three experiments; bars, SD. *P <0.05, combination treatment group compared with CDDP-alone group. E. Apoptosis rate analysis using Annexin V/PI flow cytometry in GBC-SD cells transfected with Bcl-xL plasmid for 24h before treatment with CDDP (4μg/ml)/GW4064 (5μM) combination for 48 h. Columns, mean of three experiments; bars, SD. *P <0.05, Bcl-xL/CDDP+GW4064 group compared with MOCK/CDDP+GW4064 group. F. Apoptosis rate analysis using Annexin V/PI flow cytometry in RBE cells transfected with Bcl-xL plasmid for 24h before treatment with CDDP (16μg/ml)/GW4064 (5μM) combination for 48 h. Columns, mean of three experiments; bars, SD. *P <0.05, Bcl-xL/CDDP+GW4064 group compared with MOCK/CDDP+GW4064 group.