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. 2016 Apr 27;7(23):35092–35105. doi: 10.18632/oncotarget.9042

Figure 5. MiR-199b directly targets SIRT1 in SW620 cells.

Figure 5

A. Illustration of SIRT1 3′UTR as well as the seed sequence of miR-199b showing the predicted target region on the 3′UTR of SIRT1 mRNA. B. Dual-luciferase reporter assay with cotransfection SIRT1 3′UTR plasmids and miR-199b mimics. The relative luciferase activity was obtained by Firefly luciferase activity normalized against Renilla luciferase activity. C. The effects of overexpression of miR-199b on SIRT1 expression at mRNA level and protein level. D. The effects of inhibition of miR-199b on SIRT1 expression at mRNA level and protein level. *P<0.05, **P<0.01.