Figure 2. Suppression of TCam-2 cell proliferation by DHT treatment.
A. TCam-2 cell proliferation was observed with or without DHT. 10,000 cells of TCam-2 were plated and cultured with DHT or without DHT (DHT: 10−7, 10−9 or 10−11 M) for four days. The number of living cells was examined by measurement of absorbance (450 nm) at 1, 2 and 4 days after cell seeding. Data are presented as mean ± s.d. (n=3). B. Colony formation assays using TCam-2 cells. Cells were cultured with or without DHT (DHT: 10−7 M) for 2 weeks. Colony numbers in each dish (n = 5) were visually counted and presented using a column graph. Each experiment was performed three times.* p < 0.01.