ANTIBODIES AND BISPECIFIC ANTIBODIES
Antibodies are widely used in biochemistry, molecular biology, and medical research, and one of their innovative uses has been as therapeutic agents for the treatment of a variety of diseases, including cancer. At least 45 antibody-based products are currently marketed for therapy or imaging in the United States and Europe with approximately 63 billion US dollars in total worldwide sales in 2013.1 Important advances have improved the engineering, safety, and efficacy of the first generation of therapeutic antibodies. These developments, along with a greater understanding of the immunomodulatory properties of antibodies, have paved the way for the next generation of novel and improved antibody-based therapeutics, such as bispecific antibodies (BsAbs).2-4
BsAbs combine the specificity of two antibodies so that they can simultaneously bind to different antigens.5 Most BsAbs involve specificities that bind to an antigen on a cancer cell and to T-cell surface glycoprotein CD3 ε-chain (CD3) on T cells. As with many of the strategies that are based the on use of monoclonal antibodies, BsAbs have benefited from a steady improvement in technology and lessons learned from earlier clinical and preclinical studies.6 The engineering of monospecific antibodies to be bispecific opens up a variety of potential therapeutic applications as evidenced by the more than 30 BsAbs currently in clinical development.7
BISPECIFIC T-CELL ENGAGER
A bispecific T-cell engager (BiTE) is a unique BsAb that has two linked, single-chain variable fragments constructed to be flexible and have a 1 + 1 antigen-binding valency.8 BiTEs are a class of bispecific monoclonal antibodies currently under investigation as anticancer therapeutics. They bind CD3 on T cells and an antigen on tumor cells to activate T cells to kill tumor cells. BiTEs direct a host’s immune system, more specifically the T cells’ cytotoxic activity, against cancer cells. The BiTE blinatumomab specifically targets CD19 on B cells, which is expressed throughout most of B-cell development and in corresponding B-cell malignancies. However, CD19 is not expressed on plasma cells or plasma cell neoplasias. Blinatumomab is used as a second-line treatment of Philadelphia chromosome–negative relapsed or refractory acute lymphoblastic leukemia and was approved by the US Food and Drug Administration in December 2014.
HOW DO BiTEs WORK?
BiTEs are small, flexible molecules that bring together T cells and tumor cells (Fig 1).9 They only trigger T-cell cytotoxicity and cytokine production when both binding sites are occupied.10 BiTEs activate T cells without the apparent need for costimulation, and data suggest that BiTEs preferentially activate memory T cells.11-13 Because of their small size, they are rapidly cleared through the kidneys, so continuous dosing may be needed.14-16 However, their small size may also allow more rapid tumor and tissue penetration. BiTEs are unique in that they lack an Fc-binding portion, so they do not activate Fc-bearing immune cells such as macrophages, neutrophils, or natural killer (NK) cells. Other bispecific formats may trigger NK cell cytotoxicity of tumor cells through binding to CD16a (FcRγIIIa) on NK cells instead of binding to T cells through CD3, and these are referred to as BiKEs (bispecific killer engagers).17-19
CLINICAL FINDINGS
The BiTE blinatumomab has demonstrated clinical responses at very low doses in patients with non-Hodgkin lymphoma. Because of the small protein size and rapid clearance, a continuous infusion can be used effectively, and a maximum tolerated dose of 60 μg/m2/day with an overall response rate of 69% across non-Hodgkin lymphoma subtypes has been achieved with a median response duration of 404 days.16 In contrast, intact antibodies, such as rituximab (anti-CD20), are given at doses of 375 mg/m2/day. Thus, the BiTE format allows for efficacy against tumors at very low doses.
In many patients treated with blinatumomab, mild inflammatory symptoms related to T-cell activation at initiation of therapy develops, whereas in some patients, cytokine release syndrome, a severe condition characterized by flu-like symptoms, develops.20,21 Although symptom severity varies, grade 3 or higher cytokine release syndrome has been observed in a small percentage of adult patients treated with blinatumomab.21 Release of inflammatory cytokines, such as interleukin-2 (IL-2), IL-6, IL-10, interferon gamma, and tumor necrosis factor α, has been demonstrated in both adult and pediatric patients.14,20 In the current study, the authors noticed transient proinflammatory cytokine elevations in the serum during the first 48 hours of treatment.22 Patients receiving higher doses were more likely to experience dose-limiting toxicities, primarily neurologic events.
Frequent adverse events observed include lymphopenia, pyrexia, and increased C-reactive protein concentrations, which are consistent with the mode of action of a T-cell–activating therapy that also depletes the CD19+ B cells. Another common adverse event is neurologic findings, which are believed to be due to increased cytokine release of activated T cells within the CNS, even though tumor cells may not be present in the CNS. In the study by Goebeler et al,16 corticosteroids and pentosan polysulfate were tested as methods to limit T-cell cytokine activity or trafficking of T cells into the CNS, respectively. All adverse events detected were associated with blinatumomab treatment, were time and dose dependent, and generally resolved when treatment was discontinued. Stepwise dosing of BiTEs and the addition of molecules to limit T-cell cytokine production resulted in an improved ability for patients to complete full BiTE treatment.
FUTURE DIRECTIONS
Many different BsAbs are in development. Their ability to target and activate specific immune effector cells and responses against tumors holds great promise as cancer therapy. Blinatumomab represents the first-in-class BiTE antibody in clinical use and provides a novel therapeutic option for patients with relapsed/refractory B-cell acute lymphoblastic leukemia.22-26 Protein engineering and design will be used to create molecules with powerful efficacy, and we will need to learn how to deliver doses and manage adverse events to use them effectively.
Supplementary Material
Acknowledgment
Supported in part by National Institutes of Health grant CA164178 and funds from the Center for Synthetic Immunity.
Footnotes
See accompanying article on page 1104
AUTHOR CONTRIBUTIONS
Manuscript writing: All authors
Final approval of manuscript: All authors
AUTHORS' DISCLOSURES OF POTENTIAL CONFLICTS OF INTEREST
Bispecific T-Cell Engagers (BiTEs) as Treatment of B-Cell Lymphoma
The following represents disclosure information provided by authors of this manuscript. All relationships are considered compensated. Relationships are self-held unless noted. I = Immediate Family Member, Inst = My Institution. Relationships may not relate to the subject matter of this manuscript. For more information about ASCO's conflict of interest policy, please refer to www.asco.org/rwc or jco.ascopubs.org/site/ifc.
Nicole C. Smits
No relationship to disclose
Charles L. Sentman
Stock or Other Ownership: Celyad
Honoraria: Biogen
Consulting or Advisory Role: Celdara Medical, Celdara Medical (I)
Research Funding: Celdara Medical, Celdara Medical (I, Inst)
Patents, Royalties, Other Intellectual Property: Patents on bispecific molecules and chimeric antigen receptors, patents on bispecific molecules and chimeric antigen receptors (Inst)
Travel, Accommodations, Expenses: Celdara Medical, Celyad, Biogen
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