Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1998 Feb 1;101(3):682–688. doi: 10.1172/JCI119882

Adenosine(5') oligophospho-(5') guanosines and guanosine(5') oligophospho-(5') guanosines in human platelets.

H Schlüter 1, I Grobeta 1, J Bachmann 1, R Kaufmann 1, M van der Giet 1, M Tepel 1, J R Nofer 1, G Assmann 1, M Karas 1, J Jankowski 1, W Zidek 1
PMCID: PMC508613  PMID: 9449703

Abstract

We isolated and identified nucleoside(5') oligophospho-(5') nucleosides containing adenosine and guanosine (ApnG; n = 3-6) as well as diguanosine polyphosphates (GpnG; n = 3-6) in human platelets. For identification, UV spectrometry, matrix-assisted laser desorption/ionization, postsource decay matrix-assisted laser desorption/ionization mass spectrometry, and enzymatic cleavage experiments were used. The adenosine(5') oligophospho-(5') guanosines act as vasoconstrictors and growth factors. The diguanosine polyphosphates are potent modulators of growth in vascular smooth muscle cells, but do not affect vascular tone.

Full Text

The Full Text of this article is available as a PDF (234.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aalkjaer C. Regulation of intracellular pH and its role in vascular smooth muscle function. J Hypertens. 1990 Mar;8(3):197–206. doi: 10.1097/00004872-199003000-00001. [DOI] [PubMed] [Google Scholar]
  2. Agha A., Schlüter H., König S., Biel K., Tepel M., Zidek W. A novel platelet-derived renal vasoconstrictor agent in normotensives and essential hypertensives. J Vasc Res. 1992 May-Jun;29(3):281–289. doi: 10.1159/000158943. [DOI] [PubMed] [Google Scholar]
  3. Avila D. M., Robinson A. K., Kaushal V., Barnes L. D. A paradoxical increase of a metabolite upon increased expression of its catabolic enzyme: the case of diadenosine tetraphosphate (Ap4A) and Ap4A phosphorylase I in Saccharomyces cerevisiae. J Bacteriol. 1991 Dec;173(24):7875–7880. doi: 10.1128/jb.173.24.7875-7880.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barnes L. D., Robinson A. K., Mumford C. H., Garrison P. N. Assay of diadenosine tetraphosphate hydrolytic enzymes by boronate chromatography. Anal Biochem. 1985 Jan;144(1):296–304. doi: 10.1016/0003-2697(85)90120-4. [DOI] [PubMed] [Google Scholar]
  5. Dalziel H. H., Westfall D. P. Receptors for adenine nucleotides and nucleosides: subclassification, distribution, and molecular characterization. Pharmacol Rev. 1994 Dec;46(4):449–466. [PubMed] [Google Scholar]
  6. Erlinge D., Yoo H., Edvinsson L., Reis D. J., Wahlestedt C. Mitogenic effects of ATP on vascular smooth muscle cells vs. other growth factors and sympathetic cotransmitters. Am J Physiol. 1993 Oct;265(4 Pt 2):H1089–H1097. doi: 10.1152/ajpheart.1993.265.4.H1089. [DOI] [PubMed] [Google Scholar]
  7. Erlinge D., You J., Wahlestedt C., Edvinsson L. Characterisation of an ATP receptor mediating mitogenesis in vascular smooth muscle cells. Eur J Pharmacol. 1995 Mar 15;289(1):135–149. doi: 10.1016/0922-4106(95)90178-7. [DOI] [PubMed] [Google Scholar]
  8. Fredholm B. B., Abbracchio M. P., Burnstock G., Daly J. W., Harden T. K., Jacobson K. A., Leff P., Williams M. Nomenclature and classification of purinoceptors. Pharmacol Rev. 1994 Jun;46(2):143–156. [PMC free article] [PubMed] [Google Scholar]
  9. Heidenreich S., Tepel M., Schlüter H., Harrach B., Zidek W. Regulation of rat mesangial cell growth by diadenosine phosphates. J Clin Invest. 1995 Jun;95(6):2862–2867. doi: 10.1172/JCI117992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lüthje J., Ogilvie A. Catabolism of Ap4A and Ap3A in whole blood. The dinucleotides are long-lived signal molecules in the blood ending up as intracellular ATP in the erythrocytes. Eur J Biochem. 1988 Apr 5;173(1):241–245. doi: 10.1111/j.1432-1033.1988.tb13990.x. [DOI] [PubMed] [Google Scholar]
  11. Lüthje J., Ogilvie A. Diadenosine triphosphate (Ap3A) mediates human platelet aggregation by liberation of ADP. Biochem Biophys Res Commun. 1984 Feb 14;118(3):704–709. doi: 10.1016/0006-291x(84)91451-7. [DOI] [PubMed] [Google Scholar]
  12. Lüthje J., Ogilvie A. The presence of diadenosine 5',5'''-P1,P3-triphosphate (Ap3A) in human platelets. Biochem Biophys Res Commun. 1983 Aug 30;115(1):253–260. doi: 10.1016/0006-291x(83)90997-x. [DOI] [PubMed] [Google Scholar]
  13. Malam-Souley R., Campan M., Gadeau A. P., Desgranges C. Exogenous ATP induces a limited cell cycle progression of arterial smooth muscle cells. Am J Physiol. 1993 Apr;264(4 Pt 1):C783–C788. doi: 10.1152/ajpcell.1993.264.4.C783. [DOI] [PubMed] [Google Scholar]
  14. Muallem S., Blissard D., Cragoe E. J., Jr, Sachs G. Activation of the Na+/H+ and Cl-/HCO3- exchange by stimulation of acid secretion in the parietal cell. J Biol Chem. 1988 Oct 15;263(29):14703–14711. [PubMed] [Google Scholar]
  15. Ng K. E., Orgel L. E. The action of a water-soluble carbodiimide on adenosine-5'-polyphosphates. Nucleic Acids Res. 1987 Apr 24;15(8):3573–3580. doi: 10.1093/nar/15.8.3573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ng L. L., Dudley C. Intracellular pH clamping of human leucocytes: a technique for determination of cellular buffering power and Na+/H+ antiport characteristics. Clin Sci (Lond) 1989 Oct;77(4):417–423. doi: 10.1042/cs0770417. [DOI] [PubMed] [Google Scholar]
  17. Nordhoff E., Ingendoh A., Cramer R., Overberg A., Stahl B., Karas M., Hillenkamp F., Crain P. F. Matrix-assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared. Rapid Commun Mass Spectrom. 1992 Dec;6(12):771–776. doi: 10.1002/rcm.1290061212. [DOI] [PubMed] [Google Scholar]
  18. Pintor J., Miras-Portugal M. T. A novel receptor for diadenosine polyphosphates coupled to calcium increase in rat midbrain synaptosomes. Br J Pharmacol. 1995 Jul;115(6):895–902. doi: 10.1111/j.1476-5381.1995.tb15894.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Prescott M., Milne A. D., McLennan A. G. Characterization of the bis(5'-nucleosidyl) tetraphosphate pyrophosphohydrolase from encysted embryos of the brine shrimp Artemia. Biochem J. 1989 May 1;259(3):831–838. doi: 10.1042/bj2590831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pálfi Z., Surányi G., Borbély G. Alterations in the accumulation of adenylylated nucleotides in heavy-metal-ion-stressed and heat-stressed Synechococcus sp. strain PCC 6301, a cyanobacterium, in light and dark. Biochem J. 1991 Jun 1;276(Pt 2):487–491. doi: 10.1042/bj2760487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ralevic V., Hoyle C. H., Burnstock G. Pivotal role of phosphate chain length in vasoconstrictor versus vasodilator actions of adenine dinucleotides in rat mesenteric arteries. J Physiol. 1995 Mar 15;483(Pt 3):703–713. doi: 10.1113/jphysiol.1995.sp020615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Redon J., Batlle D. Regulation of intracellular pH in the spontaneously hypertensive rat. Role of bicarbonate-dependent transporters. Hypertension. 1994 Apr;23(4):503–512. doi: 10.1161/01.hyp.23.4.503. [DOI] [PubMed] [Google Scholar]
  23. Schlüter H., Offers E., Brüggemann G., van der Giet M., Tepel M., Nordhoff E., Karas M., Spieker C., Witzel H., Zidek W. Diadenosine phosphates and the physiological control of blood pressure. Nature. 1994 Jan 13;367(6459):186–188. doi: 10.1038/367186a0. [DOI] [PubMed] [Google Scholar]
  24. Schulze-Lohoff E., Zanner S., Ogilvie A., Sterzel R. B. Extracellular ATP stimulates proliferation of cultured mesangial cells via P2-purinergic receptors. Am J Physiol. 1992 Sep;263(3 Pt 2):F374–F383. doi: 10.1152/ajprenal.1992.263.3.F374. [DOI] [PubMed] [Google Scholar]
  25. Tepel M., Klaus T., Laukemper S., Schlüter H., Zidek W. Increased lymphocytic Na+/H+ exchange activity after hemodialysis: evidence for an endogenous inhibitor of Na+/H+ exchange in patients with end-stage renal failure. Life Sci. 1996;59(18):1545–1552. doi: 10.1016/0024-3205(96)00485-7. [DOI] [PubMed] [Google Scholar]
  26. Zamecnik P. C., Stephenson M. L., Janeway C. M., Randerath K. Enzymatic synthesis of diadenosine tetraphosphate and diadenosine triphosphate with a purified lysyl-sRNA synthetase. Biochem Biophys Res Commun. 1966 Jul 6;24(1):91–97. doi: 10.1016/0006-291x(66)90415-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES