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. 1991 Feb 1;88(3):693–697. doi: 10.1073/pnas.88.3.693

Capture of flanking DNA by a P element in Drosophila melanogaster: creation of a transposable element.

S I Tsubota 1, D V Huong 1
PMCID: PMC50879  PMID: 1846960

Abstract

A 6.1-kilobase insertion into the rudimentary (r) gene was cloned and partially sequenced. The insertion consists of a 703-base-pair (bp) P element next to a 5.4-kilobase single-copy sequence. The normal position of the single-copy sequence is near the tip of the X chromosome. Upon insertion into the r gene, this chimeric element generated an 8-bp target-site duplication, characteristic of P elements. At the non-P-element end of the insertion, the first 8 bp are identical to the first 8 bp of the inverted terminal repeats of the P element. Thus, this element has inverted terminal repeats of 8 bp. This large element can excise from the r gene under conditions of hybrid dysgenesis, which indicates that it behaves like a normal P element. These data support the conclusion that a normally stable single-copy sequence has now become unstable and duplicated within the genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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